Abstract
Background: Epinephrine (EP), ascorbic acid (AA) and tyrosine (TY) usually coexist together in human blood and are considered as important molecules for physiological processes in human metabolism. Thus, to determine these compounds in biological fluids, we need to develop sensitive, simple and rapid methods.
Method: The simultaneous spectrophotometric determination of AA, EP and TY, at pH=7.0 using multivariate calibration techniques such as principal component regression (PCR), partial least squares (PLS) and their first derivative methods have been carried out.
Results: Satisfactory results were achieved using first derivative-partial least squares. After optimization of all variables, the linear ranges for AA, EP and TY were 0.8-35.0, 1.0-72.01 and 1.0-145.0 µgmL-1, respectively. The detection limits for AA, EP and TY obtained 0.1, 0.35 and 0.28 µgmL-1, respectively. Principal component (NPC) for AA, EP and TY were 2, 3 and 3. Prediction error sum of squares (PRESS) for AA, EP and TY were 2.37, 0.91 and 2.57, respectively. The root mean square errors of Prediction (RMSEP) for AA, EP and TY were 0.35, 0.16 and 0.60 respectively.
Conclusion: Good agreement between prediction and actual concentrations clearly indicates the utilization of this procedure for the simultaneous determination of AA, EP and TY in serum, plasma and urine samples and also for the determination of AA in vitamin c tablet and EP in epinephrine injection.
Keywords: UV–vis spectrophotometry, multivariate calibration, simultaneous determination, ascorbic acid, epinephrine, tyrosine.
Current Analytical Chemistry
Title:Multivariate Calibration Applied to the Simultaneous Spectrophotometric Determination of Ascorbic Acid, Tyrosine and Epinephrine in Pharmaceutical Formulation and Biological Fluids
Volume: 12 Issue: 6
Author(s): Aida Solhjoo and Habibollah Khajehsharifi
Affiliation:
Keywords: UV–vis spectrophotometry, multivariate calibration, simultaneous determination, ascorbic acid, epinephrine, tyrosine.
Abstract: Background: Epinephrine (EP), ascorbic acid (AA) and tyrosine (TY) usually coexist together in human blood and are considered as important molecules for physiological processes in human metabolism. Thus, to determine these compounds in biological fluids, we need to develop sensitive, simple and rapid methods.
Method: The simultaneous spectrophotometric determination of AA, EP and TY, at pH=7.0 using multivariate calibration techniques such as principal component regression (PCR), partial least squares (PLS) and their first derivative methods have been carried out.
Results: Satisfactory results were achieved using first derivative-partial least squares. After optimization of all variables, the linear ranges for AA, EP and TY were 0.8-35.0, 1.0-72.01 and 1.0-145.0 µgmL-1, respectively. The detection limits for AA, EP and TY obtained 0.1, 0.35 and 0.28 µgmL-1, respectively. Principal component (NPC) for AA, EP and TY were 2, 3 and 3. Prediction error sum of squares (PRESS) for AA, EP and TY were 2.37, 0.91 and 2.57, respectively. The root mean square errors of Prediction (RMSEP) for AA, EP and TY were 0.35, 0.16 and 0.60 respectively.
Conclusion: Good agreement between prediction and actual concentrations clearly indicates the utilization of this procedure for the simultaneous determination of AA, EP and TY in serum, plasma and urine samples and also for the determination of AA in vitamin c tablet and EP in epinephrine injection.
Export Options
About this article
Cite this article as:
Solhjoo Aida and Khajehsharifi Habibollah, Multivariate Calibration Applied to the Simultaneous Spectrophotometric Determination of Ascorbic Acid, Tyrosine and Epinephrine in Pharmaceutical Formulation and Biological Fluids, Current Analytical Chemistry 2016; 12 (6) . https://dx.doi.org/10.2174/1573411012999160401124820
DOI https://dx.doi.org/10.2174/1573411012999160401124820 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Advances in Microfluidic and Sensing Technologies of Biofluids.
Detection of biological fluids is an important means to assess human health and diagnose diseases. Currently, research on blood is relatively mature and widely applied in the medical field. With the development and advancement of microfluidic technology and novel detection methods, attention has turned to human physiological fluids such as ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Induction of Apoptosis by Curcumin and Its Implications for Cancer Therapy
Current Cancer Drug Targets Utility of γH2AX as a Molecular Marker of DNA Double-Strand Breaks in Nuclear Medicine: Applications to Radionuclide Therapy Employing Auger Electron-Emitting Isotopes
Current Radiopharmaceuticals Thrombospondins as Anti-Angiogenic Therapeutic Agents
Current Pharmaceutical Design Seeking the 5th Base of DNA Using Chromatographic Methods of Analysis
Current Organic Chemistry Combining Gene Therapy and Radiation Against Cancer
Current Gene Therapy Intracellular and Extracellular miRNAs in Regulation of Angiogenesis Signaling
Current Angiogenesis (Discontinued) Glutathione Transferases as Targets for Cancer Therapy
Anti-Cancer Agents in Medicinal Chemistry An Insight into Molecular Mechanisms and Novel Therapeutic Approaches in Epileptogenesis
CNS & Neurological Disorders - Drug Targets Main Isoflavones Found in Dietary Sources as Natural Anti-inflammatory Agents
Current Drug Targets Established and In-trial GPCR Families in Clinical Trials: A Review for Target Selection
Current Drug Targets PI3K/Akt/mTOR Pathway Inhibitors in Cancer: A Perspective on Clinical Progress
Current Medicinal Chemistry Advances in the Tyrosinase Inhibitors from Plant Source
Current Medicinal Chemistry Strategies for Developing Tuberculosis Vaccines: Emerging Approaches
Current Drug Targets The Inverse Relationship Between Cancer and Alzheimer's Disease: A Possible Mechanism
Current Alzheimer Research Positron Emission Tomography and Computer Tomography (PET/CT) in Prostate, Bladder, and Testicular Cancers
Current Medicinal Chemistry Emerging Insights in the Role of Tissue Factor in Cancer
Current Genomics Poor Safety and Tolerability Hamper Reaching a Potentially Therapeutic Dose in the Use of Thalidomide for Alzheimer’s Disease: Results from a Double-Blind, Placebo-Controlled Trial
Current Alzheimer Research Translational Research in Cutaneous Melanoma: New Therapeutic Perspectives
Anti-Cancer Agents in Medicinal Chemistry Theranostics of Metastatic Prostate Cancer Applying <sup>64</sup>Cu/<sup>18</sup>F/<sup>68</sup>Ga PSMA PET-CT and <sup>177</sup>Lu Radiopharmaceuticals
Current Radiopharmaceuticals The Urokinase Receptor in the Central Nervous System
CNS & Neurological Disorders - Drug Targets