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Current Analytical Chemistry

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ISSN (Print): 1573-4110
ISSN (Online): 1875-6727

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Research Article

Reed Membrane as a Novel Immobilization Matrix for the Development of an Optical Phenol Biosensor

Author(s): Yongjin Li*

Volume 16, Issue 3, 2020

Page: [316 - 320] Pages: 5

DOI: 10.2174/1573411015666190617105551

Price: $65

Abstract

Background: Biocompatible and easily available immobilization matrix is vital for the construction of enzyme-based biosensor.

Methods: Reed membrane was selected as a novel immobilization matrix to construct an optical phenol biosensor. Tyrosinase from mushroom was immobilized in a reed membrane using glutaraldehyde as a cross-linker. The detection scheme was based on the measurement of the color intensity of the adduct resulting from the reaction of 3-methyl-2-benzothiazolinone hydrazone (MBTH) with the quinone produced from the oxidation of phenol by tyrosinase. The performance of such method including specificity, sensitivity, repeatability and practical use were validated.

Results: The prepared biosensor demonstrated optimum performance at pH 6-7, temperature of 40°C and a linear response in the phenol concentration range of 5-100 μM. It also showed good operation stability for repeated measurements (over 200 times) and good storage stability after it had been kept at 4°C for 2 months.

Conclusion: Reed membrane is a novel matrix for immobilization of enzyme. The prepared biosensor permits good sensitivity, reproducibility and stability. It is anticipated that reed membrane is a promising solid support for fabricating other enzyme-based biosensors.

Keywords: Biomaterial, biosensor, enzyme immobilization, phenol detection, reed membrane, tyrosinase.

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