Abstract
In this study, a capillary electrophoresis-mass spectrometry method was applied to the determination of isoflavones in samples with low content in these analytes, such as legumes. Sample stacking was used to enhance the sensitivity of the technique. Extraction of the analytes was carried out by a modified approach of QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) methodology, in which the extracts were injected directly into electrophoretic system coupled with mass spectrometry detector. The developed method was validated and applied for the determination of isoflavone aglycones and glycosides in legume samples. The values of the validation parameters and the isoflavone contents found were compared with those obtained by LC-MS/MS. Both methods provided similar results and were comparable in terms of recovery (in the 80-120% range), precision (lower than 10 %) and limits of detection (ranging from 0.20 to 1.8 μg L-1).
Keywords: CE-ESI-MS, LC-ESI-MS/MS, Isoflavone, legume, QuEChERS.
Current Analytical Chemistry
Title:Determination of Isoflavones in Legumes by QuEChERS-Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry
Volume: 11 Issue: 2
Author(s): Lara Perez-Martin, Myriam Bustamante-Rangel and Maria Milagros Delgado-Zamarreno
Affiliation:
Keywords: CE-ESI-MS, LC-ESI-MS/MS, Isoflavone, legume, QuEChERS.
Abstract: In this study, a capillary electrophoresis-mass spectrometry method was applied to the determination of isoflavones in samples with low content in these analytes, such as legumes. Sample stacking was used to enhance the sensitivity of the technique. Extraction of the analytes was carried out by a modified approach of QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) methodology, in which the extracts were injected directly into electrophoretic system coupled with mass spectrometry detector. The developed method was validated and applied for the determination of isoflavone aglycones and glycosides in legume samples. The values of the validation parameters and the isoflavone contents found were compared with those obtained by LC-MS/MS. Both methods provided similar results and were comparable in terms of recovery (in the 80-120% range), precision (lower than 10 %) and limits of detection (ranging from 0.20 to 1.8 μg L-1).
Export Options
About this article
Cite this article as:
Perez-Martin Lara, Bustamante-Rangel Myriam and Delgado-Zamarreno Milagros Maria, Determination of Isoflavones in Legumes by QuEChERS-Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry, Current Analytical Chemistry 2015; 11 (2) . https://dx.doi.org/10.2174/1573411011999150112121608
DOI https://dx.doi.org/10.2174/1573411011999150112121608 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Advances in Microfluidic and Sensing Technologies of Biofluids.
Detection of biological fluids is an important means to assess human health and diagnose diseases. Currently, research on blood is relatively mature and widely applied in the medical field. With the development and advancement of microfluidic technology and novel detection methods, attention has turned to human physiological fluids such as ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Proteomics Studies in Oncology Towards Personalized Medicine in Public Health: Opportunities and Challenges for OMICS Research in Iran
Current Pharmacogenomics and Personalized Medicine Editorial [Hot topic: Vitamin D Deficiency in Cardiovascular and Renal Disease: New Light Shed on an Old Enemy (Guest Editor: Martin H. de Borst)]
Current Drug Targets Therapeutical Potential of CB<sub>2</sub> Receptors in Immune-Related Diseases
Current Molecular Pharmacology Oxidative Stress Genes, Antioxidants and Coronary Artery Disease in Type 2 Diabetes Mellitus
Cardiovascular & Hematological Agents in Medicinal Chemistry Cardiovascular Risk in Postmenopausal Women with Polycystic Ovary Syndrome
Current Vascular Pharmacology Oxidative Stress in HIV Patients Receiving Antiretroviral Therapy
Current HIV Research Evaluation of Plasma Homocysteine Levels as a Prognostic Factor for the Occurrence of Perioperative Myocardial Infarction in Coronary Artery Bypass Grafting: A Pilot Study
Vascular Disease Prevention (Discontinued) Triple Threat: The Na+/Ca2+ Exchanger in the Pathophysiology of Cardiac Arrhythmia, Ischemia and Heart Failure
Current Drug Targets Refined Echocardiographic Assessment and Contemporary Medical Treatment of Obstructive Hypertrophic Cardiomyopathy
Cardiovascular & Hematological Disorders-Drug Targets Resveratrol and Cardiovascular Disease
Current Nutrition & Food Science Editorial [Hot Topic: Plasminogen Activator Inhibitor-1 (Guest Editor: Daniel A. Lawrence)]
Current Drug Targets Hypertension in Egypt: A Systematic Review
Current Hypertension Reviews Serpin Regulation of Fibrinolytic System: Implications for Therapeutic Applications in Cardiovascular Diseases
Cardiovascular & Hematological Agents in Medicinal Chemistry Recent Patents on Polymeric Scaffolds for Tissue Engineering
Recent Patents on Biomedical Engineering (Discontinued) The Role of microRNAs in the Development of Type 2 Diabetes Complications
Current Pharmaceutical Design Activating Mutations of ESR1, BRCA1 and CYP19 Aromatase Genes Confer Tumor Response in Breast Cancers Treated with Antiestrogens
Recent Patents on Anti-Cancer Drug Discovery Clinical Pharmacogenetics of Methotrexate
Current Drug Metabolism A Therapeutic Target of Cerebral Hemorrhagic Stroke: Matrix Metalloproteinase- 9
Current Drug Targets Male Urogenital Disorders and Metabolic Syndrome: Possible Links, Characteristics and Potential Treatment Strategies
Current Pharmaceutical Design Purinergic Signalling and Endothelium
Current Vascular Pharmacology