Abstract
A rapid and simple microemulsion electrokinetic chromatography (MEEKC) method has been developed and validated for the determination of ethinylestradiol and levo-norgestrel in a commercial tablet formulation. The analysis was performed with an electrolyte composed of 0.5% (w/w) ethyl acetate, 1.2% (w/w) butan-1-ol, 0.6% (w/w) sodium dodecyl sulfate, 15% (v/v) ethanol and 82.7% (w/w) 12 mmol L−1 sodium tetraborate aqueous buffer at pH 9.23 and direct UV detection at 200 and 247 nm. For quantitative purposes, estrone was used as an internal standard. Retention time for levo-norgestrel and ethinylestradiol was approximately 2.1 and 2.5 min respectively. Acceptable precision (<0.90% RSD, linearity (r2 > 0.99; concentration range from 24.0 to 36.0 µg mL−1), limits of detection (LOD) and quatification (LOQ) for ethinylestradiol of 1.0 μg mL-1 and 3.2 µg mL−1, respectively, and for levo-norgestrel of 1.4 µg mL−1 and 4.1 µg mL-1, respectively, and recovery of 100 ± 2 % at three concentration levels were obtained. Based on the performance characteristics, the proposed methodology was found suitable for the determination of ethinylestradiol and levo-norgestrel in tablet formulations.
Keywords: Contraceptives, capillary electrophoresis, ethinylestradiol, levo-norgestrel, Microemulsion electrokinetic chromatography, steroids.
Current Analytical Chemistry
Title:Determination of Ethinylestradiol and Levo-Norgestrel Using Microemulsion Electrokinetic Chromatography
Volume: 10 Issue: 2
Author(s): Claudinei Alves da Silva, María Segunda Aurora-Prado and Marina Franco Maggi Tavares
Affiliation:
Keywords: Contraceptives, capillary electrophoresis, ethinylestradiol, levo-norgestrel, Microemulsion electrokinetic chromatography, steroids.
Abstract: A rapid and simple microemulsion electrokinetic chromatography (MEEKC) method has been developed and validated for the determination of ethinylestradiol and levo-norgestrel in a commercial tablet formulation. The analysis was performed with an electrolyte composed of 0.5% (w/w) ethyl acetate, 1.2% (w/w) butan-1-ol, 0.6% (w/w) sodium dodecyl sulfate, 15% (v/v) ethanol and 82.7% (w/w) 12 mmol L−1 sodium tetraborate aqueous buffer at pH 9.23 and direct UV detection at 200 and 247 nm. For quantitative purposes, estrone was used as an internal standard. Retention time for levo-norgestrel and ethinylestradiol was approximately 2.1 and 2.5 min respectively. Acceptable precision (<0.90% RSD, linearity (r2 > 0.99; concentration range from 24.0 to 36.0 µg mL−1), limits of detection (LOD) and quatification (LOQ) for ethinylestradiol of 1.0 μg mL-1 and 3.2 µg mL−1, respectively, and for levo-norgestrel of 1.4 µg mL−1 and 4.1 µg mL-1, respectively, and recovery of 100 ± 2 % at three concentration levels were obtained. Based on the performance characteristics, the proposed methodology was found suitable for the determination of ethinylestradiol and levo-norgestrel in tablet formulations.
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Cite this article as:
Silva Alves da Claudinei, Aurora-Prado Segunda María and Tavares Franco Maggi Marina, Determination of Ethinylestradiol and Levo-Norgestrel Using Microemulsion Electrokinetic Chromatography, Current Analytical Chemistry 2014; 10 (2) . https://dx.doi.org/10.2174/15734110113099990007
DOI https://dx.doi.org/10.2174/15734110113099990007 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
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