NMR Experiments for the Analysis of Mixtures: Beyond 1D 1H Spectra

Author(s): Damien Jeannerat, Julien Furrer

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 15 , Issue 1 , 2012

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State-of-the-art technologies and methodologies in NMR spectroscopy make it possible to obtain very informative and high-quality spectra in much less experimental time than classical methods by making better choices of NMR pulse sequences and acquisition parameters. This review presents some recent NMR methods allowing rapid identification, assignment and structural characterization of the components in mixtures. The relative merits of the different NMR pulse sequences are briefly discussed and recommendations are made for the preferred choice of sequences to obtain rapidly artifact-free data. This review covers diffusion experiments (DOSY), HSQC and HMBC experiments, ultra-resolved 2D spectra exploiting the property of aliasing and NOESY/ROESY experiments. It will be in particular shown that selective 1D NOESY/ROESY sequences can be more informative and reach higher resolution in less experimental time than the corresponding 2D sequences.

Keywords: Acquisition optimization, DOSY, HSQC, mixtures, NMR spectroscopy, resolution, ROESY, spectral aliasing, spectral editing, high throughput screening

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Article Details

Year: 2012
Page: [15 - 35]
Pages: 21
DOI: 10.2174/138620712798280853

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PDF: 51