Background: The growing dissatisfaction with the available traditional chemotherapeutic agents has
enhanced the need to develop new methods for obtaining materials with more effective and safe anti-cancer
properties. Over the past few years, the usage of metallic nanoparticles has been a target for researchers of
different scientific and commercial fields due to their tiny sizes, environment-friendly properties, and a wide
range of applications. To overcome the obstacles of traditional physical and chemical methods for the synthesis
of such nanoparticles, a new, less expensive, and eco-friendly method has been adopted using natural existing
organisms as a reducing agent to mediate the synthesis of the desired metallic nanoparticles from their
precursors, a process called green biosynthesis of nanoparticles.
Objective: In the present study, zinc-iron bimetallic nanoparticles (ZnFe2O4) were synthesized via an aqueous
extract of BoswelliaCarteri resin mixed with zinc acetate and iron chloride precursors, and they were tested for
their anticancer activity.
Methods: Various analytic methods were applied for the characterization of the phyto synthesized ZnFe2O4, and
they were tested for their anticancer activity against MDA-MB-231, K562, MCF-7 cancer cell lines, and normal
Results: Our results demonstrate the synthesis of cubic structured bimetallic nanoparticles ZnFe2O4 with an
average diameter of 10.54 nm. MTT cytotoxicity assay demonstrates that our phyto-synthesized ZnFe2O4
nanoparticles exhibited a selective and potent anticancer activity against K562 and MDA-MB-231 cell lines with
IC50 values 4.53 μM and 4.19 μM, respectively.
Conclusion: In conclusion, our biosynthesized ZnFe2O4 nanoparticles show a promising, environmentally
friendly, and low coast chemotherapeutic approach against selective cancers with a predicted low adverse side
effect toward normal cells. Further, in vivo, advanced animal research should be done to execute their
applicability in living organisms.