Abstract
Macrophage targeting therapies are a promising approach to treat various intracellular infections like AIDS, tuberculosis and leprosy. The present paper demonstrates the design and development of mannosylated nanoparticles of biopolymeric origin. Spherical and smooth gelatin nanoparticles in the size range of 100-200 nm with a narrow polydispersity index ( < 0.1) were synthesized and characterized by TEM, AFM and XPS for their morphology and mannosylation. Cell uptake studies by spectrofluorimetry in J774 macrophage cell-line demonstrate two-fold greater internalisation of mannosylated gelatin nanoparticles (MGNP) through macrophage mannose-receptor, than unconjugated nanoparticles (GNP); enhanced uptake of MGNP in J774 macrophages was confirmed by laser confocal microscopy. Evaluation of cellular toxicity by MTT assay reveals close to 100% cell viability confirming biocompatibility. In addition, neither MGNP nor GNP caused ROS (reactive oxidative species) generation in J774 cells. Biodistribution studies in murine model showed significant uptake of MGNP by reticuloendothelial system as early as 15 min after intravenous administration (spleen 0.99±0.14 percent injected dose/100 mg tissue; liver, 1.04±0.11 percent dose/100 mg). The anti-HIV drug stavudine was loaded to 38.2% entrapment efficiency in MGNP and showed 77.5% release in 96 h. Thus, these mannosylated nanoparticles appear to be a very useful carrier system for receptor-specific drug delivery in treatment of macrophage-mediated intracellular infections.
Keywords: Macrophage targeting, gelatin nanoparticles, mannosylation, biocompatibility, in vitro uptake, biodistribution
Current Nanoscience
Title: Receptor Specific Macrophage Targeting by Mannose-Conjugated Gelatin Nanoparticles- An In Vitro and In Vivo Study
Volume: 6 Issue: 4
Author(s): Shveta Mahajan, C.K. Prashant, Veena Koul, Veena Choudhary and Amit K. Dinda
Affiliation:
Keywords: Macrophage targeting, gelatin nanoparticles, mannosylation, biocompatibility, in vitro uptake, biodistribution
Abstract: Macrophage targeting therapies are a promising approach to treat various intracellular infections like AIDS, tuberculosis and leprosy. The present paper demonstrates the design and development of mannosylated nanoparticles of biopolymeric origin. Spherical and smooth gelatin nanoparticles in the size range of 100-200 nm with a narrow polydispersity index ( < 0.1) were synthesized and characterized by TEM, AFM and XPS for their morphology and mannosylation. Cell uptake studies by spectrofluorimetry in J774 macrophage cell-line demonstrate two-fold greater internalisation of mannosylated gelatin nanoparticles (MGNP) through macrophage mannose-receptor, than unconjugated nanoparticles (GNP); enhanced uptake of MGNP in J774 macrophages was confirmed by laser confocal microscopy. Evaluation of cellular toxicity by MTT assay reveals close to 100% cell viability confirming biocompatibility. In addition, neither MGNP nor GNP caused ROS (reactive oxidative species) generation in J774 cells. Biodistribution studies in murine model showed significant uptake of MGNP by reticuloendothelial system as early as 15 min after intravenous administration (spleen 0.99±0.14 percent injected dose/100 mg tissue; liver, 1.04±0.11 percent dose/100 mg). The anti-HIV drug stavudine was loaded to 38.2% entrapment efficiency in MGNP and showed 77.5% release in 96 h. Thus, these mannosylated nanoparticles appear to be a very useful carrier system for receptor-specific drug delivery in treatment of macrophage-mediated intracellular infections.
Export Options
About this article
Cite this article as:
Mahajan Shveta, Prashant C.K., Koul Veena, Choudhary Veena and K. Dinda Amit, Receptor Specific Macrophage Targeting by Mannose-Conjugated Gelatin Nanoparticles- An In Vitro and In Vivo Study, Current Nanoscience 2010; 6 (4) . https://dx.doi.org/10.2174/157341310791658928
DOI https://dx.doi.org/10.2174/157341310791658928 |
Print ISSN 1573-4137 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6786 |
Call for Papers in Thematic Issues
Role of nanomaterials in fabrication of coatings, Machining and Joining
The application of nanoscience has brought about a revolution in the field of mechanical engineering by providing novel materials, boosting manufacturing processes, and generating cutting-edge products. The purpose of this special issue is to investigate the enormous impact that nanoscience has had on mechanical engineering, with a particular emphasis on ...read more
Advanced Inorganic Nanocomposites and Their Emerging Applications
This special issue collection will highlight developments on the recent trends about the synthesis of metal oxides, nanoclusters, biomaterials, 2D nanomaterials, nanocrystals, nanocomposites, etc. and their applications in electrochemical systems, tissue regeneration, energy storage and harvesting, sensors, etc. The novelty of the methods in the chemical synthesis and their characterizations, ...read more
Applicability of Nanotechnology for Performance Enhancement of Clean Energy Technologies
Population growth, industrialization, and improvement in living quality would lead to further increase in energy demand in near future. Regarding the disadvantages of fossil fuels such as fluctuations in their price, high emissions of greenhouse gases and restriction of their sources, it is crucial to use and exploit alternative energy ...read more
Graphene and 2D Materials for Energy Storage and Conversion
This thematic issue will discuss the recent advances in graphene-based nanomaterials for different energy technologies. Graphene possesses a high surface area, and stable structure and exhibits many interesting electronic, optical, and mechanical properties due to its 2D crystal structure. Graphene is of both fundamental interest and suitable for a wide ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
ATP Synthase: A Molecular Therapeutic Drug Target for Antimicrobial and Antitumor Peptides
Current Medicinal Chemistry Isoniazid Induced Convulsions at Therapeutic Dose in an Alcoholic and Smoker Patient
Current Drug Safety The Role of Vitamin D in Prevention and Treatment of Infection
Inflammation & Allergy - Drug Targets (Discontinued) Editorial: [A Threat to One is a Threat to All – Biosecurity for the Western World Starts in Developing Countries: Do We Speak the Same Language of Hygiene?]
Inflammation & Allergy - Drug Targets (Discontinued) Glucocorticoids and the Cardiovascular System: State of the Art
Current Pharmaceutical Design Purine and Pyrimidine Pathways as Targets in Plasmodium falciparum
Current Topics in Medicinal Chemistry Repurposing Non-Antimicrobial Drugs and Clinical Molecules to Treat Bacterial Infections
Current Pharmaceutical Design Inhaled Formulation Design for the Treatment of Lung Infections
Current Pharmaceutical Design C-cinnamoyl Glycosides: An Emerging “Tail” for the Development of Selective Carbonic Anhydrase Inhibitors
Current Medicinal Chemistry Etiopathogenesis, Classical Immunotherapy and Innovative Nanotherapeutics for Inflammatory Neurological Disorders
Current Nanoscience Structural Bioinformatic Approaches to the Discovery of New Antimycobacterial Drugs
Current Pharmaceutical Design Maternal Sepsis 2010: Early Recognition and Aggressive Treatment with Early Goal Directed Therapy can Improve Maternal Outcomes
Current Women`s Health Reviews Phytochemical and Biological Activities of an Anticancer Plant Medicine: Brucea javanica
Anti-Cancer Agents in Medicinal Chemistry Increased Production of Recombinant O-Phospho-L-Serine Sulfhydrylase from the Hyperthermophilic Archaeon Aeropyrum pernix K1 Using Escherichia coli
Current Biotechnology HtrA Protease Family as Therapeutic Targets
Current Pharmaceutical Design Focusing on DNA Repair and Damage Tolerance Mechanisms in Mycobacterium tuberculosis: An Emerging Therapeutic Theme
Current Topics in Medicinal Chemistry Single-Dose Microparticle Delivery of a Malaria Transmission- Blocking Vaccine Elicits a Long-Lasting Functional Antibody Response
Current Molecular Medicine Death Due to COVID-19 in an Infant with Combined Immunodeficiencies
Endocrine, Metabolic & Immune Disorders - Drug Targets Prediction of Translation Initiation Site in Bacterial and Archaeal Genomes
Current Bioinformatics MIANN Models of Networks of Biochemical Reactions, Ecosystems, and U.S. Supreme Court with Balaban-Markov Indices
Current Bioinformatics