Abstract
Labeo rohita (rohu) is one of the most important freshwater carps widely cultured in India, Bangladesh and other adjacent countries in the region. Proteomic analysis from liver tissue samples of riverine L. rohita was carried out with two-dimensional polyacrylamide gel electrophoresis (2-DE). On an average 450 spots were detected in a colloidal Coomassie stained 2-DE gel using PDQuest software. Forty two spots with high abundance and clear separation were selected from the gel and twelve of them were later analyzed with Electronspray Ionization (ESI) - Mass Spectrometry and identified by MASCOT search. The paper describes first attempt for protein profiling of liver tissue samples of L. rohita based on protocol standardized in our lab for optimized yield of total protein as well as 2-D electrophoresis.
Keywords: 2-D PAGE, Labeo rohita, Mass Spectrometry, Protein expression signatures.
Current Proteomics
Title:Proteomics Analysis of Liver Tissue of Labeo rohita
Volume: 12 Issue: 1
Author(s): M. Goswami, G. Hariprasad, A. Dubey, R. Kumar, N.S. Nagpure, A. Srinivasan, T.P. Singh and W.S. Lakra
Affiliation:
Keywords: 2-D PAGE, Labeo rohita, Mass Spectrometry, Protein expression signatures.
Abstract: Labeo rohita (rohu) is one of the most important freshwater carps widely cultured in India, Bangladesh and other adjacent countries in the region. Proteomic analysis from liver tissue samples of riverine L. rohita was carried out with two-dimensional polyacrylamide gel electrophoresis (2-DE). On an average 450 spots were detected in a colloidal Coomassie stained 2-DE gel using PDQuest software. Forty two spots with high abundance and clear separation were selected from the gel and twelve of them were later analyzed with Electronspray Ionization (ESI) - Mass Spectrometry and identified by MASCOT search. The paper describes first attempt for protein profiling of liver tissue samples of L. rohita based on protocol standardized in our lab for optimized yield of total protein as well as 2-D electrophoresis.
Export Options
About this article
Cite this article as:
Goswami M., Hariprasad G., Dubey A., Kumar R., Nagpure N.S., Srinivasan A., Singh T.P. and Lakra W.S., Proteomics Analysis of Liver Tissue of Labeo rohita, Current Proteomics 2015; 12 (1) . https://dx.doi.org/10.2174/157016461201150506202445
DOI https://dx.doi.org/10.2174/157016461201150506202445 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
Call for Papers in Thematic Issues
Mass spectrometry data acquisition and analysis for proteomics
The Thematic Issue on "Mass spectrometry data acquisition and analysis for proteomics" aims to explore the latest advancements and challenges in the field of proteomics through the lens of mass spectrometry. Proteomics, the large-scale study of proteins and their functions, plays a crucial role in understanding various biological processes and ...read more
Peptides: State-of-Art and Commercialisation Hurdles
The Editors of the Current Proteomics (CP) journal are highly privileged to welcome scientists to submit their scientific research and review articles to be considered for publication in the upcoming thematic issue. The topics should cover various aspects of peptides in regard to their synthetic methodologies, formulation approaches, pharmacological challenges, ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
High Fat Diet Aggravates AD-Related Pathogenic Processes in APP/PS1 Mice
Current Alzheimer Research Imaging of Visceral Adipose Tissue: An Emerging Diagnostic Tool and Therapeutic Target
Current Drug Targets - Cardiovascular & Hematological Disorders Modified-Release Solid Formulations for Colonic Delivery
Recent Patents on Drug Delivery & Formulation Emerging Targeted Therapies for Pediatric Acute Myeloid Leukemia
Recent Patents on Anti-Cancer Drug Discovery Post-Stroke Immunodepression and Infection: An Emerging Concept
Infectious Disorders - Drug Targets Role of CNS Transporters in the Pharmacotherapy of HIV-1 Associated Neurological Disorders
Current Pharmaceutical Design Antagonists of Activated Factor X and Thrombin: Innovative Antithrombotic Agents.
Current Vascular Pharmacology Cortisol Regulation in the Metabolic Syndrome. A Novel Therapeutic Approach
Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry (Discontinued) The Association between Metabolic Syndrome and Serum Levels of Adiponectin and High Sensitive C Reactive Protein in Gorgan
Endocrine, Metabolic & Immune Disorders - Drug Targets Interrelated Roles for the Aryl Hydrocarbon Receptor and Hypoxia Inducible Factor-1α in the Immune Response to Infection
Current Immunology Reviews (Discontinued) Advances in Anticancer Antibody-Drug Conjugates and Immunotoxins
Recent Patents on Anti-Cancer Drug Discovery Surfactant Proteins in Smoking-Related Lung Disease
Current Topics in Medicinal Chemistry The Role of PrP in Health and Disease
Current Molecular Medicine Big Science for Small Cells: Systems Approaches for Platelets
Current Drug Targets Cholestasis During Pregnancy: Aetiopathogenesis, Foetal-Maternal Repercussions and Pharmacological Treatments
Current Women`s Health Reviews Transgenic Mouse Models of Alzheimer Disease: Developing a Better Model as a Tool for Therapeutic Interventions
Current Pharmaceutical Design Novel Pharmacologic Approaches to the Management of Sepsis: Targeting the Host Inflammatory Response
Recent Patents on Inflammation & Allergy Drug Discovery Possible Pathogenic Role of the Transmembrane Isoform of CD160 NK Lymphocyte Receptor in Paroxysmal Nocturnal Hemoglobinuria
Current Molecular Medicine Molecular Imaging of Therapeutic Potential of Reporter Probes
Current Drug Targets Targeting ATP7A to Increase the Sensitivity of Neuroblastoma Cells to Retinoid Therapy
Current Cancer Drug Targets