The first volume of this Ebook series brings together the most recent advances from leading experts in the burgeoning field of biotechnology. This comprehensive text adopts a multidisciplinary approach and covers agricultural biotechnology, industrial and environmental biotechnology, pharmaceutical sciences and drug developments from plant sources, biochemical and molecular studies on gene expression, forestry, genetic engineering, gene delivery approaches, plant tissue culture and biochemical techniques/methods/protocols involved in different areas of plant biotechnology. This Ebook should prove to be a valuable resource for plant biotechnologists, plant biologists, biochemists, molecular biologists, pharmacologists, and pharmacists; agronomists, plant breeders, and geneticists; ethnobotanists, ecologists, and conservationists; medical practitioners and nutritionists; research investigators in industry, federal laboratories, and universities; and students and teachers in the biological and biomedical sciences at undergraduate and postgraduate levels.

Brazil has emerged as a significant financial and industrial power in recent times. Brazil is poised to become a significant player in the field of biotechnology, internationally, by taking advantage of circumstances not available in other countries, particularly its native biodiversity. This will, in turn, have an effect on commercial and entrepreneurial opportunities in the region. Topics covered in this text include adjustments that must be made in the regulatory framework to assure the success of business investment. This investment is crucial for training R&D scientists and developing new technologies. The book also covers a debate on transgenic plants which had political ramifications in the region and slowed the adoption rate of genetically modified organisms by almost a decade. The opportunities for commercialization of recombinant DNA technologies in the country are also presented. Opportunities and Limitations For Biotechnology Innovation In Brazil presents a concise overview of the biotechnology industry in Brazil and will be of great interest to a wide range of readers including researchers, biotechnology graduates, as well as both local and international investors.

Biofuels are currently used as a viable alternative energy source in several countries. Plant-Based Genetic Tools for Biofuels Production explains biotechnological techniques and concepts that are applied to increase biofuel yield from plants and algae. Chapters of the book cover a variety of topics: the basic research techniques (cell suspension, embryogenesis, protoplast fusion), plant genetics (plant DNA mutations, new plant breeding techniques, viral genetic vectors for heterologous gene expression, sub cellular proteomes), genomic resources and bioinformatics tools, plant species with bioenergy and biofuel potential, factors influencing biomass yield, advances in cultivation technologies, fermentation of different substrates for ethanol production, and microalgae biomass technologies. Readers will gain a thorough understanding of modern biofuel production. Plant-Based Genetic Tools for Biofuels Production is a suitable reference for students in biotechnology and bioinformatics programs as well as researchers interested in information about the basics of biofuel production.

Modern techniques have been developed to overcome problems associated with the extraction of natural products from plants. These techniques include production of secondary metabolites by biotechnological methods such as plant tissue culture and microbial biotransformation of natural substances. Such methods have led to an increased yield of secondary metabolite amount, the discovery of new biochemical derivatives and agricultural development. For instance, use of these techniques in agricultural area have led to some beneficial traits such as formation of new varieties of known plant species, better crop quality, higher yield, better nutritive properties, more resistant species to insects and pests. Microbial biotransformation is beneficial in plant secondary metabolite production and derivatization (chemical modification of any compound made by a microorganism) and is chiefly applicable to several varieties of terpenes and steroids. This E-book demonstrates recent developments in this field. It will be of particular interest to the professionals in pharmaceutical and biotechnological industries, as well as natural product chemists, medicinal chemists, plant biochemists, and molecular biologists.

Bioencapsulation (or microencapsulation) of cells and their implantation into a body of immunoprotected cells allows researchers to revive a missing or defective function in the cells. Microencapsulated cells have reached the clinic where they have been shown to be safe and efficacious in numerous clinical trials. Cell types that have been encapsulated for therapeutic purposes include hybridomas, islet cells, "platform" cell lines e.g. HEK293 and CHO cells as well as an increasing focus on the encapsulation of stem cells. This e-book explains the different methods used for achieving cell bioencapsulation based on different polymers e.g. alginate, cellulose sulphate and agarose. Other chapters in this book subsequently describe the use of cell encapsulation in cancer therapy to improve on existing chemotherapies by reducing doses and thus side effects while increasing efficacy for long term in vivo production of (i) tumoricidal or virus neutralising antibodies, (ii) insulin to achieve a self regulating treatment for diabetes and (iii) neuron nurturing factors to treat CNS diseases such as epilepsy, disorders like Parkinson's disease, Alzheimer, Amyotrophic lateral sclerosis, Huntington's disease and pathologies caused by trauma and/or ischemic processes. The book also includes a chapter reviewing the genetic engineering of cells to allow controlled production of biotherapeutics from encapsulated cells. This is a unique and timely book which brings together contributions from some of the leading researchers in the field of cell encapsulation. Interested readers are provided an overview of the exciting developments and clinical experiences of researchers with cell bioencapsulation.

This authoritative reference provides update information on the fundamental aspects and applications of biomaterials and biomedical sciences. It includes nickel-titanium alloys with superelasticity and shape memory effect, bioactive bone cement for orthopedic applications, and new research trends in polymer nanocomposites. This Ebook also describes the response of cells to ionizing radiation such as DNA damage responses in zebrafish. With chapters contributed by eminent scientists in the field of biophysics, biomaterials and biomedical engineering, this is a useful reference for graduate students, researchers and professionals in biomedical sciences and technologies.

Heavy metal pollution represents a global challenge to both public health and environmental sustainability. Any means to reduce heavy metal pollution in the environment is of considerable economic significance. The use of green plants to clean up heavy metal pollution is an environmentally friendly as well as a low-cost approach to the problem. This plant-based biotechnology is commonly known as ‘phytoremediation’. Presently, there is limited application of this technology because useful plants with enhanced heavy metal resistance/tolerance are still needed to assist remediation of environments polluted with heavy metals. A key to improved phytoremediation of heavy metal pollution lies in research seeking for a better understanding of the mechanism(s) of heavy metal resistance/tolerance in plants. This E-book presents a unique treatment of the topics that have never been comprehensively brought together before in a single advanced reference. The volume explores aspects of plant biology that are critical for employing phytoremedation techniques to combat heavy metal contamination such as the specific plant biology, seed biology, plant tissue culture and enzymology. This E-book will be a useful reference to plant biologists, biotechnologists and environmental engineers seeking information about phytoremediation of heavy metals from the environment.

The reference presents detailed research on the olive (Olea europaea) and the stone fruit. Readers will learn about the biotechnology, plant nutrition, plant breeding, pomology, postharvest physiology, plant pathology of these two plant species. In a practical sense, the book also presents applicable agricultural knowledge about these plants for crop improvement, production, nutrients, pest management, disease, genetic, genomic and the food industry. The contributions by the authors of this book include descriptions about the manipulation of variables and genetic resources of inheritance of quantitative genes, crop rotation, soil water, and the effect of temperature on crop production. Aspects such as protecting crops against pests and diseases whilst ensuring the protection of human health are also taken into account. This is a valuable reference for students, scientists, horticulturists and, in general, for anyone wishing to obtain knowledge and experience with olives and drupes to increase productivity.

Carbohydrates (monosaccharides, oligosaccharides and polysaccharides) exist naturally in free from and as components of other macromolecules (nucleic acids, proteins and lipids). Glycotherapy refers to this use of carbohydrates or glycoengineered macromolecules in fighting life-threatening diseases. Although glycotherapy is a relatively new and limited field of pharmacology, significant progress has been made in recent years in academia and the pharmaceutical industry facilitated by advances in enzymology and recombinant DNA technologies. Consequently, carbohydrate containing biologics are now used in therapeutic regimens. This volume provides a summary of the progress made in developing therapeutic solutions using glycoengineering techniques. Topics covered in this volume include the development of vaccines and antibodies against tumor-associated carbohydrate antigens for cancer treatment, the use of glycan for viral inhibition, glycoPEGylation, hyaluronic acid conjugation, and protein modification and conjugation for increasing therapeutic index using recombinant and chemoenzymatic approaches. The volume is essential reading for biochemists, pharmacologists and R&D professionals interested in glycotherapy.

Global plastic production is estimated to be over 300Mt annually. Most conventional plastics are predominantly produced from fossil fuels and are highly resistant to biodegradation, and only a small share of about 20% of spent plastics is believed to be recycled, which is a cause for environmental concern. Biodegradable plastics would solve this concern as they are a sustainable alternative, yet these do not even cover 5% of the global plastic market. Microbial polyhydroxyalkanoates (PHAs) are a versatile group of polyesters produced by nature as prokaryotic storage materials. PHAs can be produced through sustainable bioprocess engineering and have displayed remarkable flexibility in their physical and chemical properties. PHAs are the subject of several scientific papers and numerous PHA patents have also been filed, generating significant interest in the plastic production industry. To develop overall sustainable and efficient production processes, all bioprocess steps need to be thoroughly understood and accounted for. These processes start with the selection of suitable inexpensive raw materials (microbes and enzymes), optimizing the process engineering and process regime, and conclude with the enhancement of product recovery in terms of time, energy, and material input. Microbial Biopolyester Production, Performance and Processing: Microbiology, Feedstocks, and Metabolism encompasses eight chapters that cover aspects of the microbiology and biotechnology of producing biodegradable plastics. The contents focus on the selection of powerful archaeal and eubacterial production strains, genetic engineering as a tool for optimized PHA production and inexpensive carbon sources for microbial cultures. The volume is a useful resource for bioprocess engineers, microbiologists, biotechnologists and chemical engineers interested in the basics of biodegradable plastic production. Recent Advances in Biotechnology is a book series comprising of peer-reviewed reference works and monographs that compile the latest developments in the field of biotechnology. Each volume has a thematic focus and features topical reviews written by experts. The series will highlight multidisciplinary perspectives to interested readers (biotechnologists, microbiologists, bioprocess engineers, agronomists, medical professionals, sustainability researchers etc.)