Abstract
Gout is a painful disease of joints generated because of the deposition of urate crystals within the joints. Uricase is an enzyme capable of degrading less water soluble urate crystals into a compound allantoin, which is more water soluble and hence is easily excreted. Uricase being proteinaceous is degraded by the protein digestive enzymes of gastro intestinal tract (GIT) when administered orally. Colonic part of GIT lacks protein digestive enzymes and thus provides suitable conditions for the absorption of uricase. Colon consists of several microbial flora which secrete enzymes capable of degradation of alginates and pectin which are not digested by upper GI tract. Eudragit S-100 (pH sensitive polymer) coated alginate – pectinate microbeads were found to be effective for delivery of uricase directly to the colonic site. Sodium taurocholate, a bile salt, was found to be suitable for the effective absorption of uricase from colonic site into the systemic circulation.
Keywords: Colon targeting, Enzyme, Enzyme therapy, Gout, Hyperuricemia, Microbeads, Uricase.
Drug Delivery Letters
Title:Fabrication and Evaluation of Colon Targeted Uricase Containing Microbeads for Treatment of Gout
Volume: 3 Issue: 1
Author(s): Harinath Dwivedi, Kusum Agarwal and Shubhini A Saraf
Affiliation:
Keywords: Colon targeting, Enzyme, Enzyme therapy, Gout, Hyperuricemia, Microbeads, Uricase.
Abstract: Gout is a painful disease of joints generated because of the deposition of urate crystals within the joints. Uricase is an enzyme capable of degrading less water soluble urate crystals into a compound allantoin, which is more water soluble and hence is easily excreted. Uricase being proteinaceous is degraded by the protein digestive enzymes of gastro intestinal tract (GIT) when administered orally. Colonic part of GIT lacks protein digestive enzymes and thus provides suitable conditions for the absorption of uricase. Colon consists of several microbial flora which secrete enzymes capable of degradation of alginates and pectin which are not digested by upper GI tract. Eudragit S-100 (pH sensitive polymer) coated alginate – pectinate microbeads were found to be effective for delivery of uricase directly to the colonic site. Sodium taurocholate, a bile salt, was found to be suitable for the effective absorption of uricase from colonic site into the systemic circulation.
Export Options
About this article
Cite this article as:
Dwivedi Harinath, Agarwal Kusum and Saraf Shubhini A, Fabrication and Evaluation of Colon Targeted Uricase Containing Microbeads for Treatment of Gout, Drug Delivery Letters 2013; 3 (1) . https://dx.doi.org/10.2174/2210304x11303010007
DOI https://dx.doi.org/10.2174/2210304x11303010007 |
Print ISSN 2210-3031 |
Publisher Name Bentham Science Publisher |
Online ISSN 2210-304X |
Call for Papers in Thematic Issues
Nanomaterial Assisted Targeted Therapies for Chronic Disorders- Preclinical to Clinical Outcomes
In recent years, the integration of nanomaterials into therapeutic strategies has emerged as a promising avenue in the pursuit of more effective treatments for chronic disorders. This dynamic field, explored in the context of "Nanomaterial Assisted Targeted Therapies for Chronic Disorders - Preclinical to Clinical Outcomes," seeks to bridge the ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Transcription, DNA Damage and Beyond: The Roles of Histone Ubiquitination and Deubiquitination
Current Protein & Peptide Science The Role of Cetuximab and Other Epidermal Growth Factor Receptor Monoclonal Antibodies in the Treatment of Advanced Non-Small Cell Lung Cancer
Reviews on Recent Clinical Trials The Role of SAPK/JNK Pathway in the Synergistic Effects of Metformin and Dacarbazine on Apoptosis in Raji and Ramos Lymphoma Cells
Current Molecular Pharmacology Mutant Cell Surface Receptors as Targets for Individualized Cancer Diagnosis and Therapy
Current Cancer Drug Targets Endogenous Enzyme-responsive Nanoplatforms for Anti-tumor Therapy
Current Drug Targets Editorial [Hot Topic: An Up-Date of Olive Oil and Bioactive Constituents in Health: Molecular Mechanisms and Clinical Implications (Executive Guest Editor: Catalina Alarcon de la Lastra Romero)]
Current Pharmaceutical Design Immunomodulation Mechanism of Antidepressants: Interactions between Serotonin/Norepinephrine Balance and Th1/Th2 Balance
Current Neuropharmacology Engineering of Exosomes: Steps Towards Green Production of Drug Delivery System
Current Drug Targets Rho Kinase and Angiogenesis
Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry (Discontinued) Clustering and Sampling of the c-Met Conformational Space: A Computational Drug Discovery Study
Combinatorial Chemistry & High Throughput Screening Synthesis and Preliminarily Cytotoxicity to A549, HCT116 and MCF-7 Cell Lines of thieno[2,3-d]pyrimidine Derivatives Containing Isoxazole Moiety
Letters in Drug Design & Discovery Microemulsions for Colorectal Cancer Treatments. General Considerations and Formulation of Methotrexate
Mini-Reviews in Medicinal Chemistry Natural Products as Exquisitely Potent Cytotoxic Payloads for Antibody- Drug Conjugates
Current Topics in Medicinal Chemistry Recent Patents on Proteasome Inhibitors of Natural Origin
Recent Patents on Anti-Cancer Drug Discovery Targeting RhoA/Rho Kinase and p21-Activated Kinase Signaling to Prevent Cancer Development and Progression
Recent Patents on Anti-Cancer Drug Discovery In Vitro Collapsing Colon Cancer Cells by Selectivity of Disulfiram-Loaded Charge Switchable Nanoparticles Against Cancer Stem Cells
Recent Patents on Anti-Cancer Drug Discovery Targeting Chk2 Kinase: Molecular Interaction Maps and Therapeutic Rationale
Current Pharmaceutical Design Vitamin D and Lung Cancer
Current Respiratory Medicine Reviews MUTYH Associated Polyposis (MAP)
Current Genomics Dendritic Cells and their Receptors in Antitumor Immune Response
Current Molecular Medicine