Abstract
Vibrational microscopy (Raman microscopy and infrared microscopy), which observes molecular vibrations, gives us the information of molecular species without staining because the observed signals are originated from intrinsic molecules of a cell. However, infrared radiation is absorbed with water, and the long wavelength (3-10 μm) limits the spatial resolution to several micrometers. Spontaneous emission of Raman scattering is quite feeble, and the Raman scattering often overlaps with one-photon fluorescence from a specimen. Coherent anti-Stokes Raman scattering (CARS) microscopy, which is one of the nonlinear Raman microscopy, is a method to overcome those problems. In this review, present system of CARS microscopy, the methods of background rejection, and applications are introduced.
Keywords: Nonlinear microscopy, optical microscopy, raman microscopy, real-time imaging, ultrafast laser
Current Pharmaceutical Biotechnology
Title:Coherent Anti-Stokes Raman Scattering Microscopy for High Speed Non- Staining Biomolecular Imaging
Volume: 14 Issue: 2
Author(s): Mamoru Hashimoto, Takeo Minamikawa and Tsutomu Araki
Affiliation:
Keywords: Nonlinear microscopy, optical microscopy, raman microscopy, real-time imaging, ultrafast laser
Abstract: Vibrational microscopy (Raman microscopy and infrared microscopy), which observes molecular vibrations, gives us the information of molecular species without staining because the observed signals are originated from intrinsic molecules of a cell. However, infrared radiation is absorbed with water, and the long wavelength (3-10 μm) limits the spatial resolution to several micrometers. Spontaneous emission of Raman scattering is quite feeble, and the Raman scattering often overlaps with one-photon fluorescence from a specimen. Coherent anti-Stokes Raman scattering (CARS) microscopy, which is one of the nonlinear Raman microscopy, is a method to overcome those problems. In this review, present system of CARS microscopy, the methods of background rejection, and applications are introduced.
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Cite this article as:
Hashimoto Mamoru, Minamikawa Takeo and Araki Tsutomu, Coherent Anti-Stokes Raman Scattering Microscopy for High Speed Non- Staining Biomolecular Imaging, Current Pharmaceutical Biotechnology 2013; 14 (2) . https://dx.doi.org/10.2174/1389201011314020004
DOI https://dx.doi.org/10.2174/1389201011314020004 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
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