Background: Oleamide is an essential substance for human health. So, the plants with high
oleamide content are great sources for health care products.
Objective: This study is conducted to investigate the quality of oleamide in plants and test the bioactivity
in the selected two studied species.
Methods: The three Ipomoea and five Dillenia species including Ipomoea alba, Ipomoea aquatica and
Ipomoea pes-caprae, and Dillenia indica, Dillenia obovata, Dillenia ovata, Dillenia parviflora and
Dillenia pentagyna were investigated for the quantity of oleamide by high-performance liquid chromatography.
The biological activity test was conducted on the powder formulation of the chosen plants,
Dillenia ovata and Dillenia parviflora at a ratio of 30:70, for anti-inflammatory activity ex vivo on a
panel of molecular targets through ion channel inhibition including voltage-gated sodium channel,
voltage-gated potassium channel, and the cardiac ion as human ether-a-go-go related gene.
Results: The results showed that the leaf extracts of I. aquatica and D. ovata gave the highest and subsequent
oleamide quantity i.e. 7.52 and 5.17 mg/g, respectively. Out of the Dillenia formulation which
contained various compounds, oleamide showed the highest percentages of inhibition at 8.0-20.0%,
and 6.2-14.2% in voltage-gated sodium channel, and voltage-gated potassium channel which had
slightly lower values than the oleamide standard, and no effect as 0.0% value inhibition in the cardiac
Conclusion: The Dillenia formulation exhibits anti-inflammatory activity without affecting the heart.
Accordingly, the three studied Ipomoea and three studied Dillenia species may be used for the same
activity as a single component or formulation with effective solvent for disease treatments.