Abstract
The membrane – peptide insertion behavior of an artificial antimicrobial peptide analogue in liposomes, planar free-standing bilayer and planar lipid membranes supported by a crystalline bacterial surface layer, termed S-layer, was investigated. The template for this peptide was peptidyl-glycine-leucine-carboxyamide (PGLa) where all lysine residues were replaced by glutamic acid resulting in a negatively charged analogue termed PGLa(-). Zeta potential measurements and calcein release experiments on liposomes revealed that the insertion of PGLa(-) can be compared to that of native antimicrobial peptides. Patch clamp recordings on free-standing lipid membranes provided evidence of pore formation at a lipid to peptide ratio (L/P) of 1600 with a single pore conductance of 25 pS. However, also a lower conductance at a high L/P (3200) was observed which might be explained by membrane disordering effects caused by PGLa(-) interaction. In line with other studies on the action of membrane active peptides, the rupture of the lipid membrane was strongly influenced by the peptide concentration. S-layer supported lipid membranes were utilized to perform combined surface-sensitive (quartz crystal microbalance with dissipation measurements) and electrical (impedance spectroscopy) measurements. These data evidenced not only the attachment and/or insertion of PGLa(-) in the supported lipid membrane but also indicated toroidal pore formation in a concentration dependent fashion. Hence, S-layer supported lipid membranes constitute a promising platform for studying the interaction and insertion of antimicrobial peptides.
Keywords: Antimicrobial peptide, electrochemical quartz crystal microbalance with dissipation monitoring, model lipid membranes, nanobiotechnology, patch-clamp, peptidyl-glycine-leucine-carboxyamide, S-layer supported bilayer.
Call for Papers in Thematic Issues
Role of nanomaterials in fabrication of coatings, Machining and Joining
The application of nanoscience has brought about a revolution in the field of mechanical engineering by providing novel materials, boosting manufacturing processes, and generating cutting-edge products. The purpose of this special issue is to investigate the enormous impact that nanoscience has had on mechanical engineering, with a particular emphasis on ...read more
Advanced Inorganic Nanocomposites and Their Emerging Applications
This special issue collection will highlight developments on the recent trends about the synthesis of metal oxides, nanoclusters, biomaterials, 2D nanomaterials, nanocrystals, nanocomposites, etc. and their applications in electrochemical systems, tissue regeneration, energy storage and harvesting, sensors, etc. The novelty of the methods in the chemical synthesis and their characterizations, ...read more
Applicability of Nanotechnology for Performance Enhancement of Clean Energy Technologies
Population growth, industrialization, and improvement in living quality would lead to further increase in energy demand in near future. Regarding the disadvantages of fossil fuels such as fluctuations in their price, high emissions of greenhouse gases and restriction of their sources, it is crucial to use and exploit alternative energy ...read more
Graphene and 2D Materials for Energy Storage and Conversion
This thematic issue will discuss the recent advances in graphene-based nanomaterials for different energy technologies. Graphene possesses a high surface area, and stable structure and exhibits many interesting electronic, optical, and mechanical properties due to its 2D crystal structure. Graphene is of both fundamental interest and suitable for a wide ...read more
Related Books
Nanoscale Field Effect Transistors: Emerging Applications
Nanoelectronics Devices: Design, Materials, and Applications Part II
Nanoelectronics Devices: Design, Materials, and Applications (Part I)
Role of Nanotechnology in Cancer Therapy
Synthesis and Applications of Semiconductor Nanostructures
Pathways to Green Nanomaterials: Plants as Raw Materials, Reducing Agents and Hosts
Applications of Nanomaterials in Medical Procedures and Treatments
Synthesis of Nanomaterials
Nanobiotechnology: Principles and Applications
Bio-Inspired Nanotechnology
3