Abstract
Nanomaterials have been utilized in biomedical applications for many years because of their unique properties such as quantum confinement, surface plasmon resonance, and superparamagnetism. These applications are expected to advance diagnosis and therapeutics. Fluorescent nanomaterials, such as quantum dots (QDs), were exalted in biological imaging and tracking, and trended to replace protein-based probes. Our previous investigation indicated that cellpenetrating peptides (CPPs) are a promising delivery system that can translocate materials efficiently in a noncovalent manner. In this study, we demonstrate that arginine-rich CPPs can noncovalently complex with QDs and significantly raise efficiency of cellular entry. We further examined their mechanisms of cellular penetrations, subcellular localizations, and cytotoxicity. Importantly, CPP/QD complexes were not toxic at the level of efficient transduction. Collectively, our study provided an insight that CPPs can facilitate the delivery of nanomaterials into cells. Various compositions of CPPs are a major factor affecting uptake routes and efficiency for drug delivery applications.
Keywords: Cell-penetrating peptides, pharmacological inhibitors, polyarginine, protein transduction, quantum dots.
Pharmaceutical Nanotechnology
Title:Cellular Internalization of Quantum Dots Mediated by Cell-Penetrating Peptides
Volume: 1 Issue: 2
Author(s): Betty Revon Liu, Huey-Jenn Chiang, Yue-Wern Huang, Ming-Huan Chan, Hwei-Hsien Chen and Han-Jung Lee
Affiliation:
Keywords: Cell-penetrating peptides, pharmacological inhibitors, polyarginine, protein transduction, quantum dots.
Abstract: Nanomaterials have been utilized in biomedical applications for many years because of their unique properties such as quantum confinement, surface plasmon resonance, and superparamagnetism. These applications are expected to advance diagnosis and therapeutics. Fluorescent nanomaterials, such as quantum dots (QDs), were exalted in biological imaging and tracking, and trended to replace protein-based probes. Our previous investigation indicated that cellpenetrating peptides (CPPs) are a promising delivery system that can translocate materials efficiently in a noncovalent manner. In this study, we demonstrate that arginine-rich CPPs can noncovalently complex with QDs and significantly raise efficiency of cellular entry. We further examined their mechanisms of cellular penetrations, subcellular localizations, and cytotoxicity. Importantly, CPP/QD complexes were not toxic at the level of efficient transduction. Collectively, our study provided an insight that CPPs can facilitate the delivery of nanomaterials into cells. Various compositions of CPPs are a major factor affecting uptake routes and efficiency for drug delivery applications.
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Cite this article as:
Revon Liu Betty, Chiang Huey-Jenn, Huang Yue-Wern, Chan Ming-Huan, Chen Hwei-Hsien and Lee Han-Jung, Cellular Internalization of Quantum Dots Mediated by Cell-Penetrating Peptides, Pharmaceutical Nanotechnology 2013; 1 (2) . https://dx.doi.org/10.2174/2211738511301020010
DOI https://dx.doi.org/10.2174/2211738511301020010 |
Print ISSN 2211-7385 |
Publisher Name Bentham Science Publisher |
Online ISSN 2211-7393 |
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Polymeric nanocarriers in drug delivery
Polymeric nanocarriers play a crucial role in drug delivery due to their versatility, and unique properties for targeting and modifying drug release. Their ability to enhance therapeutic outcomes, reduce side effects, and enable the delivery of drugs in a more targeted and controlled manner made them popular in the last ...read more
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