Abstract
The herpes simplex virus (HSV) is a widespread human pathogen and for many reasons the development of anti-herpetic drugs from natural products has been encouraged. Adenanthera pavonina (Ap) is a medicinal plant widely used in Brazil, among other uses. Herein, a native Ap seed polysaccharide (PLSAp) and its chemically sulfated derivate (SPLSAp) were studied by Fourier transform IR spectra (FT-IR), gel permeation chromatography (GPC) for molar mass determination and their intrinsic viscosity [η]. Biologically, the compounds were evaluated for anti-HSV activity, in HEp2 cell cultures. The cytotoxic concentrations (CC50) and the inhibitory concentrations (IC50) of the polysaccharides were determined by the colorimetric assay (dimethyl-thiazolyl-diphenyltetrazolium bromide) and plaque reduction assay (PRA), respectively. The SPLSAp showed a better antiviral activity when compared to the PLSAp with a CC50 of 500 μg/ml, the IC50 equal to 15μg/ml and the selectivity index (SI) of 33.3. The time-of-addition and the time-of-removal assays demonstrated the highest inhibitory activity between 8-16h after the infection. The inhibition of viral DNA and protein syntheses by SPLSAp monitored by PCR and immunofluorescence assay (IFA), respectively, has also demonstrated. These findings demonstrated that the SPLSAp inhibited HSV-1 infection in different steps of the replication and, therefore, represents a valuable compound for preclinical studies in anti-herpetic therapy.
Keywords: Adenanthera pavonina, Antiviral, HSV, HEp-2 cells, Physical chemistry, Polysaccharides.
Current Pharmaceutical Biotechnology
Title:Characterization and antiherpetic activity of native and chemically sulfated polysaccharide from Adenanthera pavonina
Volume: 16 Issue: 11
Author(s): Ananda M. de Godoi, Ligia C. Faccin-Galhardi, Nayara Lopes, Carlos Nozawa, Raimundo R. de Almeida, Nagila M.P.S. Ricardo and Rosa E.C. Linhares
Affiliation:
Keywords: Adenanthera pavonina, Antiviral, HSV, HEp-2 cells, Physical chemistry, Polysaccharides.
Abstract: The herpes simplex virus (HSV) is a widespread human pathogen and for many reasons the development of anti-herpetic drugs from natural products has been encouraged. Adenanthera pavonina (Ap) is a medicinal plant widely used in Brazil, among other uses. Herein, a native Ap seed polysaccharide (PLSAp) and its chemically sulfated derivate (SPLSAp) were studied by Fourier transform IR spectra (FT-IR), gel permeation chromatography (GPC) for molar mass determination and their intrinsic viscosity [η]. Biologically, the compounds were evaluated for anti-HSV activity, in HEp2 cell cultures. The cytotoxic concentrations (CC50) and the inhibitory concentrations (IC50) of the polysaccharides were determined by the colorimetric assay (dimethyl-thiazolyl-diphenyltetrazolium bromide) and plaque reduction assay (PRA), respectively. The SPLSAp showed a better antiviral activity when compared to the PLSAp with a CC50 of 500 μg/ml, the IC50 equal to 15μg/ml and the selectivity index (SI) of 33.3. The time-of-addition and the time-of-removal assays demonstrated the highest inhibitory activity between 8-16h after the infection. The inhibition of viral DNA and protein syntheses by SPLSAp monitored by PCR and immunofluorescence assay (IFA), respectively, has also demonstrated. These findings demonstrated that the SPLSAp inhibited HSV-1 infection in different steps of the replication and, therefore, represents a valuable compound for preclinical studies in anti-herpetic therapy.
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de Godoi M. Ananda, Faccin-Galhardi C. Ligia, Lopes Nayara, Nozawa Carlos, de Almeida R. Raimundo, Ricardo M.P.S. Nagila and Linhares E.C. Rosa, Characterization and antiherpetic activity of native and chemically sulfated polysaccharide from Adenanthera pavonina, Current Pharmaceutical Biotechnology 2015; 16 (11) . https://dx.doi.org/10.2174/1389201016666150731111013
DOI https://dx.doi.org/10.2174/1389201016666150731111013 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
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