Abstract
On-line HPLC-NMR chemical profiling of crude extracts derived from the bulbs of the Australian carnivorous plant Drosera erythrohiza subspecies magna was undertaken. A combination of on-flow and stop-flow HPLC-NMR methodologies enabled partial identification of two structurally related naphthoquinones as well as a mixture of their corresponding glycosides. Subsequent semi-preparative HPLC off-line isolation followed by NMR spectroscopic and mass spectrometric analysis of crude extracts of the bulbs of the plant permitted the unequivocal identification of droserone, hydroxydroserone, droserone-5-O-β-glycoside and hydroxydroserone-5-O-β-glycoside. In addition off-line separation methodologies of the aerial parts of the plant led to the identification of the bioactive compound plumbagin. This study represents only the second application of HPLC-NMR to chemically profile crude extracts of an Australian plant.
Keywords: Carnivorous plant, chemical profiling, droseraceae, drosera, glycosides, HPLC-NMR, naphthoquinones, natural products
The Natural Products Journal
Title:HPLC-NMR Chemical Profiling of the Australian Carnivorous Plant, Drosera erythrohiza subspecies magna
Volume: 3 Issue: 1
Author(s): Michael Anthony Timmers, Daniel Anthony Dias and Sylvia Urban
Affiliation:
Keywords: Carnivorous plant, chemical profiling, droseraceae, drosera, glycosides, HPLC-NMR, naphthoquinones, natural products
Abstract: On-line HPLC-NMR chemical profiling of crude extracts derived from the bulbs of the Australian carnivorous plant Drosera erythrohiza subspecies magna was undertaken. A combination of on-flow and stop-flow HPLC-NMR methodologies enabled partial identification of two structurally related naphthoquinones as well as a mixture of their corresponding glycosides. Subsequent semi-preparative HPLC off-line isolation followed by NMR spectroscopic and mass spectrometric analysis of crude extracts of the bulbs of the plant permitted the unequivocal identification of droserone, hydroxydroserone, droserone-5-O-β-glycoside and hydroxydroserone-5-O-β-glycoside. In addition off-line separation methodologies of the aerial parts of the plant led to the identification of the bioactive compound plumbagin. This study represents only the second application of HPLC-NMR to chemically profile crude extracts of an Australian plant.
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Cite this article as:
Anthony Timmers Michael, Anthony Dias Daniel and Urban Sylvia, HPLC-NMR Chemical Profiling of the Australian Carnivorous Plant, Drosera erythrohiza subspecies magna, The Natural Products Journal 2013; 3 (1) . https://dx.doi.org/10.2174/2210315511303010008
DOI https://dx.doi.org/10.2174/2210315511303010008 |
Print ISSN 2210-3155 |
Publisher Name Bentham Science Publisher |
Online ISSN 2210-3163 |
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