Expression, Renaturation and Functional Analysis of an Excitatory Insect-Specific Toxin from Scorpion Buthus martensii Karsch

Title: Expression, Renaturation and Functional Analysis of an Excitatory Insect-Specific Toxin from Scorpion Buthus martensii Karsch

Volume: 12 Issue: 7

Author(s): Chong Li, Wei Liu, Frank Bossmans, Rong-Huan Zhu, Jan Tytget and Da-Cheng Wang

Affiliation:

Keywords: bmk it-ap, e. coli, recombinant expression, denaturation and renaturation

Abstract: The cDNA of BmK IT-AP, an excitatory insect toxin from the scorpion Buthus martensi Karsch that has an analgesic effect on mammalian cells, was expressed in E. coli in the form of an inclusion body. Following denaturation and reduction, the recombinant protein was renatured and purified by liquid chromatography. The authenticity of the recombinant product was confirmed by bioassay and its electrophysiological effect on insect sodium channel.

Cite this article as:

Li Chong, Liu Wei, Bossmans Frank, Zhu Rong-Huan, Tytget Jan and Wang Da-Cheng, Expression, Renaturation and Functional Analysis of an Excitatory Insect-Specific Toxin from Scorpion Buthus martensii Karsch, Protein & Peptide Letters 2005; 12 (7) . https://dx.doi.org/10.2174/0929866054696172