Abstract
In soybean, a small hormone like peptide, leginsulin was found to bind Basic 7S Globulin (Bg7S) and stimulate its tyrosine kinase activity. The NMR-structure of leginsulin, along with crystal structure of Bg7S, was used to create a rigid docking model, followed by flexible refinement of the interaction complex. This study provides structural insights into the binding of leginsulin to Bg7S. The complex is stabilized predominantly by hydrophobic forces and hydrogen bonds. The cleft is lined to a large extent by the cysteine rich region of the α-subunit of chain D as well as the carboxylterminal region of α-subunit of chain A. The model is in agreement with the available experimental evidence for the hotspot regions of Bg7S and leginsulin. We also observed another similar site on the surface of Bg7S by virtue of its pseudo- 222 symmetry. We have further hypothesized that two leginsulin molecules may bind to Bg7S in its crystal structure or tetrameric form.
Keywords: Soybean, Leginsulin, Basic 7S Globulin, 4kDa peptide, Protein-peptide docking, Tyrosine kinase activity.
Letters in Drug Design & Discovery
Title:Interaction between Basic 7S Globulin and Leginsulin in Soybean [Glycine max]: A Structural Insight
Volume: 11 Issue: 2
Author(s): Amandeep Singh, Prasoon Kumar Thakur, Megha Meena, Dhiraj Kumar, Sonika Bhatnagar, Ashok K. Dubey and Md. Imtaiyaz Hassan
Affiliation:
Keywords: Soybean, Leginsulin, Basic 7S Globulin, 4kDa peptide, Protein-peptide docking, Tyrosine kinase activity.
Abstract: In soybean, a small hormone like peptide, leginsulin was found to bind Basic 7S Globulin (Bg7S) and stimulate its tyrosine kinase activity. The NMR-structure of leginsulin, along with crystal structure of Bg7S, was used to create a rigid docking model, followed by flexible refinement of the interaction complex. This study provides structural insights into the binding of leginsulin to Bg7S. The complex is stabilized predominantly by hydrophobic forces and hydrogen bonds. The cleft is lined to a large extent by the cysteine rich region of the α-subunit of chain D as well as the carboxylterminal region of α-subunit of chain A. The model is in agreement with the available experimental evidence for the hotspot regions of Bg7S and leginsulin. We also observed another similar site on the surface of Bg7S by virtue of its pseudo- 222 symmetry. We have further hypothesized that two leginsulin molecules may bind to Bg7S in its crystal structure or tetrameric form.
Export Options
About this article
Cite this article as:
Singh Amandeep, Kumar Thakur Prasoon, Meena Megha, Kumar Dhiraj, Bhatnagar Sonika, K. Dubey Ashok and Imtaiyaz Hassan Md., Interaction between Basic 7S Globulin and Leginsulin in Soybean [Glycine max]: A Structural Insight, Letters in Drug Design & Discovery 2014; 11 (2) . https://dx.doi.org/10.2174/15701808113109990060
DOI https://dx.doi.org/10.2174/15701808113109990060 |
Print ISSN 1570-1808 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-628X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Molecular Mechanisms of Epigenetic Regulators as Activatable Targets in Cancer Theranostics
Current Medicinal Chemistry The Changing Face of HDAC Inhibitor Depsipeptide
Current Cancer Drug Targets ETS Proteins and MMPs: Partners in Invasion and Metastasis
Current Drug Targets Oxidative and Nitrosative Stress and Immune-inflammatory Pathways in Patients with Myalgic Encephalomyelitis (ME)/Chronic Fatigue Syndrome (CFS)
Current Neuropharmacology Indoleamine 2,3-dioxygenase, Tregs and Cancer
Current Medicinal Chemistry Heparan Sulfate Proteoglycans, Tumour Progression and the Cancer Stem Cell Niche
Current Cancer Therapy Reviews “Click Chemistry” for Molecular Imaging
Current Molecular Imaging (Discontinued) Multiple Mechanisms of Cytokine Action in Neurodegenerative and Psychiatric States: Neurochemical and Molecular Substrates
Current Pharmaceutical Design Isolation, Purification and Characterization of a Novel Steroidal Saponin Cholestanol Glucoside from Lasiodiplodia theobromae that Induces Apoptosis in A549 Cells
Anti-Cancer Agents in Medicinal Chemistry NAD+-Linked 15-Hydroxyprostaglandin Dehydrogenase: Structure and Biological Functions
Current Pharmaceutical Design Suicide Gene Therapy Mediated by the Herpes Simplex Virus Thymidine Kinase Gene / Ganciclovir System: Fifteen Years of Application
Current Gene Therapy Pre-feasibility Study for Establishing Radioisotope and Radiopharmaceutical Production Facilities in Developing Countries
Current Radiopharmaceuticals Recent Knowledge and New Pharmaceutical Products in Potential Alleviation of Endometriosis
Recent Patents on Inflammation & Allergy Drug Discovery Pharmacological Modulation of Nitric Oxide Release: New Pharmacological Perspectives, Potential Benefits and Risks
Current Medicinal Chemistry Aurora A and B Kinases - Targets of Novel Anticancer Drugs
Recent Patents on Anti-Cancer Drug Discovery Endocrine and Antineoplastic Actions of Growth Hormone-Releasing Hormone Antagonists
Current Medicinal Chemistry Therapeutic Approach to Multiple Sclerosis by Novel Oral Drugs
Recent Patents on Inflammation & Allergy Drug Discovery Nanoparticle-Based Tumor Theranostics with Molecular Imaging
Current Pharmaceutical Biotechnology The Holy Grail of Polymer Therapeutics for Cancer Therapy: An Overview on the Pharmacokinetics and Bio Distribution
Current Drug Metabolism Targeting Membrane Receptors of Ovarian Cancer Cells for Therapy
Current Cancer Drug Targets