Abstract
Protein arrays represent a step forward in proteomic technologies, as they enable to address biological and functional questions by large-scale approaches (i.e., up to several thousand proteins may be monitored in a single experiment). Here we review recent achievements of protein arrays and their classification. We also summarize current and future applications of this technology to address the study of the human proteome including protein identification, biomarker discovery, screening of protein-protein/molecule interactions, detection of protein post-translational modifications and serum profiling. Advantages and limitations of protein arrays compared to other proteomic technologies are also discussed here. The use, advances and recent applications of fluorescent microspheres as a promising alternative to planar arrays are also summarized in this report. Finally, we propose future perspectives of protein arrays from a comprehensive standpoint that includes technological, biological, economical and practical aspects.
Keywords: Advantages and limitations of protein arrays, antibody characterization, array classification, cell-free protein synthesis, human proteome, microspheres, nanoparticles, NAPPA, perspectives, protein arrays, proteomics.
Current Proteomics
Title:Protein Arrays: Recent Achievements and their Application to Study the Human Proteome
Volume: 10 Issue: 2
Author(s): Juan Casado-Vela, Maria Gonzalez-Gonzalez, Sergio Matarraz, Maria Jose Martinez-Esteso, Maite Vilella, Jose Maria Sayagues, Manuel Fuentes and Juan Carlos Lacal
Affiliation:
Keywords: Advantages and limitations of protein arrays, antibody characterization, array classification, cell-free protein synthesis, human proteome, microspheres, nanoparticles, NAPPA, perspectives, protein arrays, proteomics.
Abstract: Protein arrays represent a step forward in proteomic technologies, as they enable to address biological and functional questions by large-scale approaches (i.e., up to several thousand proteins may be monitored in a single experiment). Here we review recent achievements of protein arrays and their classification. We also summarize current and future applications of this technology to address the study of the human proteome including protein identification, biomarker discovery, screening of protein-protein/molecule interactions, detection of protein post-translational modifications and serum profiling. Advantages and limitations of protein arrays compared to other proteomic technologies are also discussed here. The use, advances and recent applications of fluorescent microspheres as a promising alternative to planar arrays are also summarized in this report. Finally, we propose future perspectives of protein arrays from a comprehensive standpoint that includes technological, biological, economical and practical aspects.
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Cite this article as:
Casado-Vela Juan, Gonzalez-Gonzalez Maria, Matarraz Sergio, Jose Martinez-Esteso Maria, Vilella Maite, Maria Sayagues Jose, Fuentes Manuel and Carlos Lacal Juan, Protein Arrays: Recent Achievements and their Application to Study the Human Proteome, Current Proteomics 2013; 10 (2) . https://dx.doi.org/10.2174/1570164611310020003
DOI https://dx.doi.org/10.2174/1570164611310020003 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
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The Thematic Issue on "Mass spectrometry data acquisition and analysis for proteomics" aims to explore the latest advancements and challenges in the field of proteomics through the lens of mass spectrometry. Proteomics, the large-scale study of proteins and their functions, plays a crucial role in understanding various biological processes and ...read more
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