Comparison of the Diagnostic Performance of Antigen B Purified from Sheep Hydatid Cyst Fluid (HCF) with Commercial ELISA Kit

Title:Comparison of the Diagnostic Performance of Antigen B Purified from Sheep Hydatid Cyst Fluid (HCF) with Commercial ELISA Kit

Volume: 24

Author(s): Fatemeh Abdollahpour Darzi, Hossein Asgarian-Omran, Shahabeddin Sarvi, Reza Valadan, Maryam Hataminejad, Sabah Mayahi, Seyyed Ali Shariatzadeh, Takatom Abbasi, Tahereh Mikaeili Galeh, Mahdi Fakhar, Majid Fassihi Harandi and Shirzad Gholami*

Affiliation:

• Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran
• Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Keywords: B antigen, Cystic Echinococcosis, Echinococcus granulosus, ELISA, Hydatid cyst, Serological method

Abstract: Introduction: Cystic echinococcosis (CE) is a zoonotic parasitic disease caused by the metacestode of Echinococcus granulosus. CE is a health problem in Middle Eastern countries, such as Iran. The purpose of this study was to purify subunit 8 KDa antigen B from crude sheep hydatid cyst fluid (HCF) and compare its sensitivity and specificity with a commercial human ELISA kit (PT-Hydatid-96).

Methods: 28 sera samples were collected from hydatid cyst patients who had surgery for a hydatid cyst and had their disease confirmed by pathology after the surgery. Furthermore, 35 samples of healthy individuals with no history of hydatid cysts were collected, as were nine serum samples from parasite-infected non-CE patients. HCF was obtained from sheep fertile cysts at a Sari slaughterhouse and used as an antigen. In an indirect ELISA test, the B antigen was employed, and the results were compared to those from a commercial ELISA kit.

Results: The results of this study were analyzed using the Kappa test. The commercial ELISA kit showed 17 cases (23.6%) positive, 44 cases (61.1%) negative, and 11 cases (15.3%) borderline. B antigen showed that 18 (25%), 43 (59.7 %), and 11 (15.3%) were positive, negative, and borderline, respectively. One sample (1.4% of 72 total samples) of 35 serum samples from healthy individuals was positive using B antigen-based ELISA. In addition, all nine serum samples from parasite-infected non-CE patients were negative for both tests. The sensitivity and specificity of the commercial ELISA kit have been evaluated at 60.7% and 100%, respectively. For B antigenbased ELISA, these values are 64.3 and 97.7%, respectively.

Conclusion: Antigen B produced from hydatid cyst fluid is a promising option for serological identification of hydatid cysts in both infected and healthy individuals. In an indirect ELISA test, hydatid fluid antigen could be used as a precise source of detection.

Cite this article as:

Darzi Abdollahpour Fatemeh, Asgarian-Omran Hossein, Sarvi Shahabeddin, Valadan Reza, Hataminejad Maryam, Mayahi Sabah, Shariatzadeh Ali Seyyed, Abbasi Takatom, Galeh Mikaeili Tahereh, Fakhar Mahdi, Harandi Fassihi Majid and Gholami Shirzad*, Comparison of the Diagnostic Performance of Antigen B Purified from Sheep Hydatid Cyst Fluid (HCF) with Commercial ELISA Kit, Infectious Disorders - Drug Targets 2024; 24 () : e200224227165 . https://dx.doi.org/10.2174/0118715265281114240131045945