Abstract
Background: In the paper, Boletus Brucella was used as carbon source material to prepare carbon dots (CDs) by one-step hydrothermal method. The CDs had high quantum yield and high photostability.
Methods: A range of characterization studies were conducted on CDs, and the results showed that the average particle size of CDs was 5 nm, emitting blue fluorescence. The optimal excitation wavelength was 337 nm, and the emission wavelength was 440 nm.
Results: Based on the static quenching, the fluorescence of CDs could be effectively quenched by VB2. Therefore, a highly sensitive and selective fluorescent probe for detecting VB2 was constructed. The CDs were successfully used to detect tablets, human blood, and urine.
Conclusion: The recovery rate of VB2 was 97.55~99.45%, and the relative standard deviation was 1.29~3.76 (n=3).
Keywords: Keywords: carbon dots, drug, analysis and detection, static quenching
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