Login Register Cart 0
Generic placeholder image

Current Chemical Biology

Editor-in-Chief

ISSN (Print): 2212-7968
ISSN (Online): 1872-3136

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

PEGylated DPPC/Anti-SNAP25 Antibody Targeted Liposomes from Langmuir Monolayer Study to Formulations

Author(s): Lai Ti Gew* and Misni Misran

Volume 15, Issue 3, 2021

Published on: 04 August, 2021

Page: [249 - 261] Pages: 13

DOI: 10.2174/2212796815666210804111958

Price: $65

Abstract

Background: Molecule compatibility is an important factor to be considered before preparing antibody-targeted liposomes, stealth-liposomes, and stealth antibody-targeted liposomes.

Objective: To determine the intermolecular interaction of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamide- N-[methoxy(polyethyleneglycol)-2000] (ammonium salt), DOPE PEG2000 and Anti-SNAP25 (AS25) in 1,2-dipalmitoyl-sn-glycero-3-phosphorylcholine (DPPC) monolayer, and their liposomes.

Methods: In this study, DPPC was used to create a monolayer mimicking the half membrane of liposomes to investigate its interactions with a polyclonal antibody, AS25, and DOPE PEG2000, which are based on Langmuir-Blodgett (LB) techniques. The surface morphology of DPPC-AS25 and DPPC-DOPE PEG2000-AS25 bilayers were also imaged and analyzed by using atomic force microscopy (AFM) to support the LB findings. The LB findings were then utilized as a reference to prepare DPPC liposomes in this work.

Results: The best mole ratio of DPPC-DOPE PEG2000, determined to be 50 to 1, was used to study the interaction with the polyclonal antibody AS25. The free energy of mixing (ΔGmix) of DPPC- DOPE PEG2000-AS25 was more negative than DPPC-AS25 in the entire investigated ranges, indicating that the ternary mixture of DPPC-DOPE PEG2000-AS25 was more compatible than the binary mixture of DPPC-AS25. The presence of DOPE PEG2000 in DPPC-AS25 increased the fluidity of the membrane, which resulted in a greater interaction of AS25 with DPPC.

Conclusion: The constant values of particle size and zeta potential measurements of DPPC-DOPE PEG2000-AS25 liposomes showed agreement with the LB findings, indicating that LB is a good technique to predict precise liposomal formulations.

Keywords: Intermolecular interactions, membrane fluidity, drug delivery, stealth liposomes, DPPC/Anti-SNAP25 antibody, Langmuir-Blodgett (LB).

« Previous Next »
Graphical Abstract

[1]
Elderdfi M, Sikorski AF. Langmuir-monolayer methodologies for characterizing protein-lipid interactions. Chem Phys Lipids 2018; 212: 61-72.
[http://dx.doi.org/10.1016/j.chemphyslip.2018.01.008] [PMID: 29360431]
[2]
Li J, Wang X, Zhang T, Wang C, Huang Z, Luo X. A review on phospholipids and their main applications in drug delivery systems. Asian J Pharm Sci 2015; 10(2): 81-98.
[http://dx.doi.org/10.1016/j.ajps.2014.09.004]
[3]
Harayama T, Riezman H. Understanding the diversity of membrane lipid composition. Nat Rev Mol Cell Biol 2018; 19(5): 281-96.
[http://dx.doi.org/10.1038/nrm.2017.138] [PMID: 29410529]
[4]
Pisal DS, Kosloski MP, Balu-Iyer SV. Delivery of therapeutic proteins. J Pharm Sci 2010; 99(6): 2557-75.
[http://dx.doi.org/10.1002/jps.22054] [PMID: 20049941]
[5]
Weiss A, Neuberg P, Philippot S, Erbacher P, Weill CO. Intracellular peptide delivery using amphiphilic lipid-based formulations. Biotechnol Bioeng 2011; 108(10): 2477-87.
[http://dx.doi.org/10.1002/bit.23182] [PMID: 21520021]
[6]
Palm W, Rodenfels J. Understanding the role of lipids and lipoproteins in development. Development 2020; 147(24): dev186411.
[http://dx.doi.org/10.1242/dev.186411] [PMID: 33355243]
[7]
Corradi V, Sejdiu BI, Mesa-Galloso H, et al. Emerging diversity in lipid–protein interactions. Chem Rev 2019; 119(9): 5775-848.
[http://dx.doi.org/10.1021/acs.chemrev.8b00451] [PMID: 30758191]
[8]
Stefaniu C, Brezesinski G, Möhwald H. Langmuir monolayers as models to study processes at membrane surfaces. Adv Colloid Interface Sci 2014; 208: 197-213.
[http://dx.doi.org/10.1016/j.cis.2014.02.013] [PMID: 24612663]
[9]
Szcześ A, Jurak M, Chibowski E. Stability of binary model membranes- prediction of the liposome stability by the Langmuir monolayer study. J Colloid Interface Sci 2012; 372(1): 212-6.
[http://dx.doi.org/10.1016/j.jcis.2012.01.035] [PMID: 22326232]
[10]
Di J, Xie F, Xu Y. When liposomes met antibodies: Drug delivery and beyond. Adv Drug Deliv Rev 2020; 154-155: 151-62.
[http://dx.doi.org/10.1016/j.addr.2020.09.003] [PMID: 32926944]
[11]
Saraf S, Jain A, Tiwari A, Verma A, Panda PK, Jain SK. Advances in liposomal drug delivery to cancer: An overview. J Drug Deliv Sci Technol 2020; 56: 101549.
[http://dx.doi.org/10.1016/j.jddst.2020.101549]
[12]
Baek S, Phan MD, Lee J, Shin K. Packing effects on polymerization of diacetylene lipids in liposomes and monolayers matrices. Polym J 2016; 48(4): 457-63.
[http://dx.doi.org/10.1038/pj.2015.136]
[13]
Chen J, Lu WL, Gu W, et al. Drug-in-cyclodextrin-in-liposomes: A promising delivery system for hydrophobic drugs. Expert Opin Drug Deliv 2014; 11(4): 565-77.
[http://dx.doi.org/10.1517/17425247.2014.884557] [PMID: 24490763]
[14]
Gew LT, Suk VRE, Misran M. Preparation and characterization of pegylated C18 fatty acids/anti-SNAP25 antibody-targeted liposomes. Curr Chem Biol 2019; 13(2): 129-39.
[http://dx.doi.org/10.2174/2212796812666180912113156]
[15]
Gew LT, Misran M. Energetic mixing of anti-SNAP25 on lipid monolayers: degree of saturation of C18 fatty acids. Surf Interface Anal 2017; 49(5): 388-97.
[http://dx.doi.org/10.1002/sia.6144]
[16]
Gew LT, Misran M. Interaction between C18 fatty acids and DOPE PEG2000 in Langmuir monolayers: effect of degree of unsaturation. J Biol Phys 2017; 43(3): 397-414.
[http://dx.doi.org/10.1007/s10867-017-9459-2] [PMID: 28752254]
[17]
Gew LT, Misran M. Mixed Langmuir monolayers of C18 fatty acids: effect of degree of saturation. Mater Sci Forum 2020; 990: 117-23.
[http://dx.doi.org/10.4028/www.scientific.net/MSF.990.117]
[18]
Gentine P, Bourel-Bonnet L, Frisch B. Modified and derived ethanol injection toward liposomes: development of the process. J Liposome Res 2013; 23(1): 11-9.
[http://dx.doi.org/10.3109/08982104.2012.717298] [PMID: 23020802]
[19]
Jaafar-Maalej C, Diab R, Andrieu V, Elaissari A, Fessi H. Ethanol injection method for hydrophilic and lipophilic drug-loaded liposome preparation. J Liposome Res 2010; 20(3): 228-43.
[http://dx.doi.org/10.3109/08982100903347923] [PMID: 19899957]
[20]
Pons M, Foradada M, Estelrich J. Liposomes obtained by the ethanol injection method. Int J Pharm 1993; 95(1-3): 51-6.
[http://dx.doi.org/10.1016/0378-5173(93)90389-W]
[21]
Hąc-Wydro K, Dynarowicz-Łątka P. Interaction between nystatin and natural membrane lipids in Langmuir monolayers-the role of a phospholipid in the mechanism of polyenes mode of action. Biophys Chem 2006; 123(2-3): 154-61.
[http://dx.doi.org/10.1016/j.bpc.2006.05.015] [PMID: 16766114]
[22]
Kamilya T, Pal P, Talapatra GB. Interaction of ovalbumin with phospholipids Langmuir-Blodgett film. J Phys Chem B 2007; 111(5): 1199-205.
[http://dx.doi.org/10.1021/jp063377l] [PMID: 17266275]
[23]
Hąc-Wydro K, Dynarowicz-Łatka P, Grzybowska J, Borowski E. Interactions of amphotericin B derivative of low toxicity with biological membrane components- the Langmuir monolayer approach. Biophys Chem 2005; 116(1): 77-88.
[http://dx.doi.org/10.1016/j.bpc.2005.03.001] [PMID: 15911084]
[24]
Hąc-Wydro K, Kapusta J, Jagoda A, Wydro P, Dynarowicz-Łatka P. The influence of phospholipid structure on the interactions with nystatin, a polyene antifungal antibiotic A Langmuir monolayer study. Chem Phys Lipids 2007; 150(2): 125-35.
[http://dx.doi.org/10.1016/j.chemphyslip.2007.06.222] [PMID: 17681287]
[25]
Castoldi A, Herr C, Niederstraber J, et al. Calcifediol-loaded liposomes for local treatment of pulmonary bacterial infections. Eur J Pharm Biopharm 2017; 118: 62-7.
[http://dx.doi.org/10.1016/j.ejpb.2016.11.026] [PMID: 27888144]
[26]
Askarizadeh A, Jaafari MR, Khamesipour A, Badiee A. Liposomal adjuvant development for leishmaniasis vaccines. Ther Adv Vaccines 2017; 5(4-5): 85-101.
[http://dx.doi.org/10.1177/2051013617741578] [PMID: 29201374]
[27]
Chibowski E, Szcześ A. Zeta potential and surface charge of DPPC and DOPC liposomes in the presence of PLC enzyme. Adsorption 2016; 22(4-6): 755-65.
[http://dx.doi.org/10.1007/s10450-016-9767-z]
[28]
Lin M-W, Huang YB, Chen CL, et al. A formulation study of 5-aminolevulinic encapsulated in DPPC liposomes in melanoma treatment. Int J Med Sci 2016; 13(7): 483-9.
[http://dx.doi.org/10.7150/ijms.15411] [PMID: 27429584]

Rights & Permissions Print Cite
Article Metrics
9
2
1
© 2024 Bentham Science Publishers | Privacy Policy