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Current Drug Research Reviews


ISSN (Print): 2589-9775
ISSN (Online): 2589-9783

Research Article

Suberosin Attenuates the Proliferation of MCF-7 Breast Cancer Cells in Combination with Radiotherapy or Hyperthermia

Author(s): Saeedeh Jafari Nodooshan, Peyman Amini, Milad Ashrafizadeh, Saeed Tavakoli, Tayebeh Aryafar, Leila Khalafi, Ahmed Eleojo Musa, Seyed Rabie Mahdavi, Masoud Najafi*, Akbar Ahmadi* and Bagher Farhood*

Volume 13, Issue 2, 2021

Published on: 27 December, 2020

Page: [148 - 153] Pages: 6

DOI: 10.2174/2589977512666201228104528

Price: $65


Aim: The aim of this study was to determine the proliferation of MCF-7 following irradiation or hyperthermia as alone or pre-treatment with suberosin.

Background: Radiotherapy is a major therapeutic modality for the control of breast cancer. However, hyperthermia can be prescribed for relief of pain or enhancing cancer cell death. Some studies have attempted its use as an adjuvant to improve therapeutic efficiency. Suberosin is a cumarin- derived natural agent that has shown anti-inflammatory properties.

Objective: In this in vitro study, possible sensitization effect of suberosin in combination with radiation or hyperthermia was evaluated.

Methods: MCF-7 breast cancer cells were irradiated or received hyperthermia with or without treatment with suberosin. The incidence of apoptosis as well as viability of MCF-7 cells were observed. Furthermore, the expressions of pro-apoptotic genes such as Bax, Bcl-2, and some caspases were evaluated using real-time PCR.

Results: Both radiotherapy or hyperthermia reduced the proliferation of MCF-7 cells. Suberosin amplified the effects of radiotherapy or hyperthermia for induction of pro-apoptotic genes and reducing cell viability.

Conclusion: Suberosin has a potent anti-cancer effect when combined with radiotherapy or hyperthermia. It could be a potential candidate for killing breast cancer cells as well as increasing the therapeutic efficiency of radiotherapy or hyperthermia.

Keywords: Suberosin, MCF-7, breast cancer, radiation, hyperthermia, radiotherapy, apoptosis.

Graphical Abstract
DeSantis CE, Ma J, Gaudet MM, et al. Breast cancer statistics, 2019. CA Cancer J Clin 2019; 69(6): 438-51.
[] [PMID: 31577379]
Ghoncheh M, Pournamdar Z, Salehiniya H. Incidence and mortality and epidemiology of breast cancer in the world. Asian Pac J Cancer Prev 2016; 17(S3): 43-6.
[] [PMID: 27165206]
Toraya-Brown S, Fiering S. Local tumour hyperthermia as immunotherapy for metastatic cancer. Int J Hyperthermia 2014; 30(8): 531-9.
[] [PMID: 25430985]
Gee HE, Moses L, Stuart K, et al. Contouring consensus guidelines in breast cancer radiotherapy: comparison and systematic review of patterns of failure. J Med Imaging Radiat Oncol 2019; 63(1): 102-15.
[] [PMID: 30267561]
Ahmed K, Tabuchi Y, Kondo T. Hyperthermia: an effective strategy to induce apoptosis in cancer cells. Apoptosis 2015; 20(11): 1411-9.
[] [PMID: 26354715]
Ariyafar T, Mahdavi SR, Geraily G, et al. Evaluating the effectiveness of combined radiotherapy and hyperthermia for the treatment response of patients with painful bony metastases: a phase 2 clinical trial. J Therm Biol 2019; 84: 129-35.
[] [PMID: 31466745]
Jeziorski K. Hyperthermia in rheumatic diseases. A promising approach? Reumatologia 2018; 56(5): 316-20.
[] [PMID: 30505014]
Mortezaee K, Najafi M, Farhood B, Ahmadi A, Shabeeb D, Musa AE. NF-κB targeting for overcoming tumor resistance and normal tissues toxicity. J Cell Physiol 2019; 234(10): 17187-204.
[] [PMID: 30912132]
Rupnow BA, Knox SJ. The role of radiation-induced apoptosis as a determinant of tumor responses to radiation therapy. Apoptosis 1999; 4(2): 115-43.
[] [PMID: 14634289]
Sarath VJ, So CS, Won YD, Gollapudi S. Artemisia princeps var orientalis induces apoptosis in human breast cancer MCF-7 cells. Anticancer Res 2007; 27(6B): 3891-8.
[PMID: 18225547]
Prietsch RF, Monte LG, da Silva FA, et al. Genistein induces apoptosis and autophagy in human breast MCF-7 cells by modulating the expression of proapoptotic factors and oxidative stress enzymes. Mol Cell Biochem 2014; 390(1-2): 235-42.
[] [PMID: 24573886]
Yin S-Y, Wei W-C, Jian F-Y, Yang N-S. Therapeutic applications of herbal medicines for cancer patients. Evid-Based Complementary Altern Med 2013; 2013: 302426.
Golfakhrabadi F, Abdollahi M, Ardakani MR, et al. Anticoagulant activity of isolated coumarins (suberosin and suberenol) and toxicity evaluation of Ferulago carduchorum in rats. Pharm Biol 2014; 52(10): 1335-40.
[] [PMID: 25017518]
Kiziltas H, Ekin S, Bayramoglu M, et al. Antioxidant properties of Ferulago angulata and its hepatoprotective effect against N-nitrosodimethylamine-induced oxidative stress in rats. Pharm Biol 2017; 55(1): 888-97.
[] [PMID: 28142310]
Farzaei F, Heydarpour F, Farzaei M, Haghighi Z, Abbasabadi Z, Sadeghi E. Protective effect of Ferulago angulata (Schltdl.) Boiss. hydroalcoholic extract against acetic acid-induced colitis in rat: Role of pro-inflammatory cytokines. Pharmacognosy Res 2018; 10(4): 391-6.
Sajjadi SE, Eskandarian A-A, Shokoohinia Y, et al. Antileishmanial activity of prenylated coumarins isolated from Ferulago angulata and Prangos asperula. Res Pharm Sci 2016; 11(4): 324-31.
[] [PMID: 27651813]
Lee H, Park HJ, Park C-S, et al. Response of breast cancer cells and cancer stem cells to metformin and hyperthermia alone or combined. PLoS One 2014; 9(2): e87979.
[] [PMID: 24505341]
Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001; 29(9): e45-5.
[] [PMID: 11328886]
Smith BD, Bellon JR, Blitzblau R, et al. Radiation therapy for the whole breast: Executive summary of an American Society for Radiation Oncology (ASTRO) evidence-based guideline. Pract Radiat Oncol 2018; 8(3): 145-52.
[] [PMID: 29545124]
Mortezaee K, Parwaie W, Motevaseli E, et al. Targets for improving tumor response to radiotherapy. Int Immunopharmacol 2019; 76: 105847.
[] [PMID: 31466051]
Ribeiro AAL, da Silva FH, de Melo Cotrim AC, et al. Herbal mixture adsorbed to polyethylene glycol microspheres induces apoptotic effects on breast cancer cells. Curr Drug Deliv 2018; 15(2): 227-34.
[] [PMID: 28969566]
Peña-Blanco A, García-Sáez AJ. Bax, Bak and beyond - mitochondrial performance in apoptosis. FEBS J 2018; 285(3): 416-31.
[] [PMID: 28755482]
Buchholz TA, Garg AK, Chakravarti N, et al. The nuclear transcription factor kappaB/bcl-2 pathway correlates with pathologic complete response to doxorubicin-based neoadjuvant chemotherapy in human breast cancer. Clin Cancer Res 2005; 11(23): 8398-402.
[] [PMID: 16322301]
Chen YC, Tsai WJ, Wu MH, Lin LC, Kuo YC. Suberosin inhibits proliferation of human peripheral blood mononuclear cells through the modulation of the transcription factors NF-AT and NF-kappaB. Br J Pharmacol 2007; 150(3): 298-312.
[] [PMID: 17179947]
Niaudet C, Bonnaud S, Guillonneau M, et al. Plasma membrane reorganization links acid sphingomyelinase/ceramide to p38 MAPK pathways in endothelial cells apoptosis. Cell Signal 2017; 33: 10-21.
[] [PMID: 28179144]
Zhu G-H, Dai H-P, Shen Q, Ji O, Zhang Q, Zhai Y-L. Curcumin induces apoptosis and suppresses invasion through MAPK and MMP signaling in human monocytic leukemia SHI-1 cells. Pharm Biol 2016; 54(8): 1303-11.
[PMID: 26134921]
Mortezaee K, Najafi M, Farhood B, Ahmadi A, Shabeeb D, Musa AE. Resveratrol as an adjuvant for normal tissues protection and tumor sensitization. Curr Cancer Drug Targets 2020; 20(2): 130-45.
[PMID: 31738153]

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