Mitochondria-Targeted Green Fluorescent Protein for Quantitative Monitoring of Mitochondrial Morphology in Living Cells

Author(s): Yasutomo Nomura, Toshifumi Takayama, Michihiko Sato, Eiji Takahashi, Zhonggang Feng, Takao Nakamura

Journal Name: Letters in Drug Design & Discovery

Volume 6 , Issue 5 , 2009

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Abstract:

In addition to Rhodamine 123, a progress in genetic engineering allowed the microscopic observation of mitochondria with GFP (mtGFP). Here we evaluated these labeling procedures for their ability to analyze mitochondrial morphology quantitatively. Expression of mtGFP was the optimal procedure to monitor mitochondrial morphology quantitatively, because of photo-stability and precise localization.

Keywords: Mitochondria, GFP, RH123, Image processing, Photobleaching

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Article Details

VOLUME: 6
ISSUE: 5
Year: 2009
Page: [319 - 322]
Pages: 4
DOI: 10.2174/1570180810906050319
Price: $65

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