Background: Bacterial cellulose (BC) has been extensively utilized in a wide range of applications
specifically in the biomedical field thanks to its excellent physico-chemical and biological features. The major
limitation restricting its application in certain areas is its high production cost. Its widespread applications demand
exploration of alternative production media compared to the existing expensive ones. Herein, an effort has
been made to utilize waste and cheaply available local resources including; waste (expired) orange juice (WOJ),
sugarcane juice (SC) and coconut water (CW) as alternative media for BC production in comparison to the synthetic
Methods: Waste and cheap resources were collected from the local market, screened filtered and optimized for
the development of BC culture media. BC production from all media was observed under static cultivation for 10
days. The results indicated 2.75, 2.56, 3.32 and 1.68 g/L BC production that corresponded to 27.5%, 21.7 %, 20.1
% and 31.6 % sugar to BC conversion from control, WOJ, SC and CW media, respectively. Morphology and
crystalline features of produced BC samples were observed through FE-SEM and XRD analysis. It was noteworthy
that BC produced from all alternative sources indicated high water holding capabilities (WHC) and water
retention time (WRT) that augment their applicability in drug delivery and wound healing applications.
Conclusion: The BC production from cheap resources and its high physical, mechanical and biological properties
can be of high interest for scaling up and commercialization of BC production processes. Furthermore, its liquidabsorbing
capabilities and retention time can help in drug carrying and medical application.