A Simple Extraction Procedure for HPLC Analysis of Fluoxetine in Rat Plasma Samples

Author(s): Tuba Recber, Ece Ozkan, Emine Eren-Kocak, Murat Yılmaz, Emirhan Nemutlu, Sedef Kır

Journal Name: Current Pharmaceutical Analysis

Volume 13 , Issue 1 , 2017

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Graphical Abstract:


Introduction: In present study, a simple liquid-liquid extraction-high performance liquid chromatography–fluorescence method was developed for the determination of fluoxetine (FLU) in rat plasma samples.

Materials and Methods: A MZ Analytical Column C18 (250 mm × 4.6 mm, 5µm i.d.) was used for the separation of FLU. The total analysis time was less than 7 minutes. The rat plasma samples were simply treated with acetonitrile and analyzed using developed method.

Conclusion: The recovery of the FLU from rat plasma samples is 92.70% ± 0.48. FLU in spiked rat plasma samples provided linear data over a range of 0.2 – 2.00 µg mL-1. The method was validated according to the FDA guidelines and can be proposed for quantification of FLU in rat plasma.

Keywords: Fluoxetine, high performance liquid chromatography, rat plasma, validation, fluorescence detection.

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Article Details

Year: 2017
Published on: 24 November, 2016
Page: [80 - 84]
Pages: 5
DOI: 10.2174/1573412912666160422153616
Price: $65

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