A Simple Extraction Procedure for HPLC Analysis of Fluoxetine in Rat Plasma Samples

Title:A Simple Extraction Procedure for HPLC Analysis of Fluoxetine in Rat Plasma Samples

Volume: 13 Issue: 1

Author(s): Tuba Recber, Ece Ozkan, Emine Eren-Kocak, Murat Yılmaz, Emirhan Nemutlu and Sedef Kır

Affiliation:

Keywords: Fluoxetine, high performance liquid chromatography, rat plasma, validation, fluorescence detection.

Abstract:

Introduction: In present study, a simple liquid-liquid extraction-high performance liquid chromatography–fluorescence method was developed for the determination of fluoxetine (FLU) in rat plasma samples.

Materials and Methods: A MZ Analytical Column C18 (250 mm × 4.6 mm, 5µm i.d.) was used for the separation of FLU. The total analysis time was less than 7 minutes. The rat plasma samples were simply treated with acetonitrile and analyzed using developed method.

Conclusion: The recovery of the FLU from rat plasma samples is 92.70% ± 0.48. FLU in spiked rat plasma samples provided linear data over a range of 0.2 – 2.00 µg mL-1. The method was validated according to the FDA guidelines and can be proposed for quantification of FLU in rat plasma.

Cite this article as:

Recber Tuba, Ozkan Ece, Eren-Kocak Emine, Yılmaz Murat, Nemutlu Emirhan and Kır Sedef, A Simple Extraction Procedure for HPLC Analysis of Fluoxetine in Rat Plasma Samples, Current Pharmaceutical Analysis 2017; 13 (1) . https://dx.doi.org/10.2174/1573412912666160422153616