Abstract
Many applications in biomedical research require the long-term identification and tracking of cells over time. In previous work we have demonstrated that by sequentially dosing a cell population with different emission wavelength nanoparticles it is possible to use the random number of nanoparticle loaded vesicles generated by the cells as a barcode for individual cells within the population. In this paper we develop a simple model to describe the number of codes that can be generated using this sequential loading protocol. The methodology is validated by comparison with experiment and subsequently used to predict the effect of varying the number of colors used to encode the cells and also to assess the effect of misreading the cellular code due to errors in imaging the vesicles.
Keywords: Microscopy, nanoparticles, time-lapse, barcoding.
Combinatorial Chemistry & High Throughput Screening
Title:An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding
Volume: 19 Issue: 5
Author(s): Paul Rees, M. Rowan Brown, John W. Wills and Huw Summers
Affiliation:
Keywords: Microscopy, nanoparticles, time-lapse, barcoding.
Abstract: Many applications in biomedical research require the long-term identification and tracking of cells over time. In previous work we have demonstrated that by sequentially dosing a cell population with different emission wavelength nanoparticles it is possible to use the random number of nanoparticle loaded vesicles generated by the cells as a barcode for individual cells within the population. In this paper we develop a simple model to describe the number of codes that can be generated using this sequential loading protocol. The methodology is validated by comparison with experiment and subsequently used to predict the effect of varying the number of colors used to encode the cells and also to assess the effect of misreading the cellular code due to errors in imaging the vesicles.
Export Options
About this article
Cite this article as:
Rees Paul, Rowan Brown M., W. Wills John and Summers Huw, An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding, Combinatorial Chemistry & High Throughput Screening 2016; 19 (5) . https://dx.doi.org/10.2174/1386207319666160408150649
DOI https://dx.doi.org/10.2174/1386207319666160408150649 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
Call for Papers in Thematic Issues
Artificial Intelligence Methods for Biomedical, Biochemical and Bioinformatics Problems
Recently, a large number of technologies based on artificial intelligence have been developed and applied to solve a diverse range of problems in the areas of biomedical, biochemical and bioinformatics problems. By utilizing powerful computing resources and massive amounts of data, methods based on artificial intelligence can significantly improve the ...read more
Emerging trends in diseases mechanisms, noble drug targets and therapeutic strategies: focus on immunological and inflammatory disorders
Recently infectious and inflammatory diseases have been a key concern worldwide due to tremendous morbidity and mortality world Wide. Recent, nCOVID-9 pandemic is a good example for the emerging infectious disease outbreak. The world is facing many emerging and re-emerging diseases out breaks at present however, there is huge lack ...read more
Exploring Spectral Graph Theory in Combinatorial Chemistry
Scope of the Thematic Issue: Combinatorial chemistry involves the synthesis and analysis of a large number of diverse compounds simultaneously. Traditional methods rely on brute force experimentation, which can be time-consuming and resource-intensive. Spectral Graph Theory, a branch of mathematics dealing with the properties of graphs in relation to the ...read more
Integrating Network Pharmacology and Traditional Medicine: A New Perspective in Drug Mechanism Research
Network pharmacology is a network construction and network topology analysis strategy that combines pharmacology and pharmacodynamics. In recent years, network pharmacology has emerged as a powerful tool that can be integrated with pharmacology. Natural products commonly function in multicomponent, multitarget, and multipathway systems. Some examples encompass Ayurveda, traditional Chinese medicines ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Pulmonary Manifestations of Rheumatoid Arthritis, Psoriatic Arthritis and Peripheral Spondyloarthritis: Prevalence, Diagnostic Approach and Treatment Options
Current Rheumatology Reviews Roles of Casein Kinase I η and δ in Gastrointestinal Cancers: Potential New Screening Markers and Drug Targets
Current Cancer Therapy Reviews 18F-Labeled Proteins
Current Pharmaceutical Biotechnology Docetaxel/2-Hydroxypropyl β -Cyclodextrin Inclusion Complex Increases Docetaxel Solubility and Release from a Nanochannel Drug Delivery System
Current Drug Targets Near-infrared Light Activatable Multimodal Gold Nanostructures Platform: An Emerging Paradigm for Cancer Therapy
Current Cancer Drug Targets Inflammasome Signaling in Pathogenesis of Lung Diseases
Current Pharmaceutical Design Comparison of the Structure and Biological Activities of Wild-type and Mutant Liver-targeting Peptide Modified Recombinant Human Endostatin (rES-CSP) in Human Hepatocellular Carcinoma HepG2 Cells
Protein & Peptide Letters Host Pharmacogenetics in the Treatment of HIV and Cancer
Current Drug Safety Getting a Handle on RAS-targeted Therapies: Cysteine Directed Inhibitors
Mini-Reviews in Medicinal Chemistry New Cathepsin D Inhibitor Library Utilizing Hydroxyethyl Isosteres with Cyclic Tertiary Amines
Medicinal Chemistry Subject Index To Volume 6
Current Topics in Medicinal Chemistry Polymers and Drug Delivery Systems
Current Drug Delivery Realizing the Potential of Blueberry as Natural Inhibitor of Metastasis and Powerful Apoptosis Inducer: Tapping the Treasure Trove for Effective Regulation of Cell Signaling Pathways
Anti-Cancer Agents in Medicinal Chemistry Activation of Large Form Galanin-LI by Extracellular Processing in Small Cell Lung Carcinoma Tissue
Protein & Peptide Letters MicroRNA-mediated Regulation of Angiogenesis
Current Angiogenesis (Discontinued) Src Family Kinases: Potential Targets for the Treatment of Human Cancer and Leukemia
Current Pharmaceutical Design Reconceptualizing Adult Neurogenesis: Role for Sphingosine-1-Phosphate and Fibroblast Growth Factor-1 in Co-Ordinating Astrocyte-Neuronal Precursor Interactions
CNS & Neurological Disorders - Drug Targets Rational Drug Design for Identifying Novel Multi-target Inhibitors for Hepatocellular Carcinoma
Anti-Cancer Agents in Medicinal Chemistry Overview of Current Immunotherapies Targeting Mutated KRAS Cancers
Current Topics in Medicinal Chemistry Development of Radicicol Analogues
Current Cancer Drug Targets