Abstract
5-Hydroxytyptophan is a fluorescent tryptophan analog that can be incorporated into recombinant proteins expressed in E. coli and is particularly useful in studies of biological systems that involve supermolecular aggregates of more than one protein. Here we review the varied applications of 5-hydroxytryptophan to study structure, interactions, conformational change and dynamics in complex protein systems.
Keywords: 5-hydroxytryptophan, tryptophan, fluorescence, protein structure, protein-protein interactions, protein folding
Protein & Peptide Letters
Title: Using 5-Hydroxytryptophan as a Probe to Follow Protein-Protein Interactions and Protein Folding Transitions
Volume: 12 Issue: 3
Author(s): Fernando Correa and Chuck S. Farah
Affiliation:
Keywords: 5-hydroxytryptophan, tryptophan, fluorescence, protein structure, protein-protein interactions, protein folding
Abstract: 5-Hydroxytyptophan is a fluorescent tryptophan analog that can be incorporated into recombinant proteins expressed in E. coli and is particularly useful in studies of biological systems that involve supermolecular aggregates of more than one protein. Here we review the varied applications of 5-hydroxytryptophan to study structure, interactions, conformational change and dynamics in complex protein systems.
Export Options
About this article
Cite this article as:
Correa Fernando and Farah S. Chuck, Using 5-Hydroxytryptophan as a Probe to Follow Protein-Protein Interactions and Protein Folding Transitions, Protein & Peptide Letters 2005; 12 (3) . https://dx.doi.org/10.2174/0929866053587057
DOI https://dx.doi.org/10.2174/0929866053587057 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Identifying Endogenous Neural Stem Cells in the Adult Brain In Vitro and In Vivo: Novel Approaches
Current Pharmaceutical Design Integrins and Cancer: Gene Expression, Epigenetics and Metastasis
Current Genomics Current Treatment Strategies for Non-Alcoholic Fatty Liver Disease (NAFLD)
Current Drug Discovery Technologies Potential Anti-cancer Drugs Commonly Used for Other Indications
Current Cancer Drug Targets The Role of Niacin in Lipid-lowering Treatment: Are we Aiming Too High?
Current Pharmaceutical Design Clinical Uses of 1,25-dihydroxy-19-nor-vitamin D<sub>2</sub> (Paricalcitol)
Current Vascular Pharmacology MiRNA-145 and Its Direct Downstream Targets in Digestive System Cancers: A Promising Therapeutic Target
Current Pharmaceutical Design Long Term Stability Evaluation of Prostacyclin Released from Biomedical Device through Turbiscan Lab Expert
Medicinal Chemistry Nocturia in Obstructive Sleep Apnea-Hypopnea Syndrome: An Underappreciated Symptom
Current Respiratory Medicine Reviews Adrenomedullin: Roles for Structure and Function in Cardiac or Vascular Tissues
Current Hypertension Reviews Thioredoxin System Modulation by Plant and Fungal Secondary Metabolites
Current Medicinal Chemistry Chronic Kidney Disease and Cardiovascular Disease: Is there Any Relationship?
Current Cardiology Reviews Polyethylene Glycol Acts as a Mechanistic Stabilizer of L-asparaginase: A Computational Probing
Medicinal Chemistry Islet Amyloid Polypeptide (IAPP): A Second Amyloid in Alzheimer's Disease
Current Alzheimer Research 11β-Hydroxysteroid Dehydrogenase Type 1: A Potential Therapeutic Target for the Control of Local Glucocorticoid Concentrations
Current Enzyme Inhibition Ultra-micronized Palmitoylethanolamide Effects on Sleep-wake Rhythm and Neuropathic Pain Phenotypes in Patients with Carpal Tunnel Syndrome: An Open-label, Randomized Controlled Study
CNS & Neurological Disorders - Drug Targets Drug Repositioning for Treatment of Movement Disorders: From Serendipity to Rational Discovery Strategies
Current Topics in Medicinal Chemistry Viral Anti-Inflammatory Reagents: The Potential for Treatment of Arthritic and Vasculitic Disorders
Endocrine, Metabolic & Immune Disorders - Drug Targets Induced Pluripotent Stem Cells in Regenerative Medicine and Disease Modeling
Current Stem Cell Research & Therapy The Structural Similarity of Predicted Chickens MHC Regulatory Factors and Bacterial Organisms
Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry (Discontinued)