Abstract
Asparagine can be non-enzymatically deamidated and isomerized via succinimide to isoaspartate. This posttranslational modification can potentially alter the physical properties or the function of the parent protein. Asn32 of ribonuclease U2A from Ustilago sphaerogena is known to rapidly deamidate and isomerize in alkaline conditions. The crystal structure of ribonuclease U2A complexed with 2´-adenylic acid and calcium ions was determined at 1.03 Å resolution. In this structure, the region from Asp29 to Asp37 winds around a calcium ion, and the main-chain of Asn32 – Gly33 adopts an extended conformation. Rotation of the side-chain of Asn32 could bring Asn32Cγ into close proximity to Gly33N, in a conformation suitable for succinimide formation. The structure suggests that in solution the region around Asn32 – Gly33 is likely to be in equilibrium between multiple conformers, with the deamidation of Asn32 proceeding when the region adopts an extended conformation.
Keywords: Asparagine deamidation, isoaspartate, isomerization, ribonuclease U2, succinimide, Ustilago sphaerogena, Ca Ion, 2'-Adenylic Acid, Asparagine, Asn32, ribonuclease U2A, Asp29, Asp37, Asn32Cγ, isoAsp, adenosine 2'-adenylic acid, (2'-AMP), polyethylene glycol, X-ray diffraction, glycerol, glycosyl bond, C2'-endo ribose pucker, Tyr39, His41, Glu62, Arg85, His101, Asp29Oδ1, Val30O, Asn32Oδ1, Asp37Oδ
Protein & Peptide Letters
Title: Conformational Variation Revealed by the Crystal Structure of Rnase U2A Complexed with Ca Ion and 2´-Adenylic Acid at 1.03 Å Resolution
Volume: 17 Issue: 12
Author(s): Shuji Noguchi
Affiliation:
Keywords: Asparagine deamidation, isoaspartate, isomerization, ribonuclease U2, succinimide, Ustilago sphaerogena, Ca Ion, 2'-Adenylic Acid, Asparagine, Asn32, ribonuclease U2A, Asp29, Asp37, Asn32Cγ, isoAsp, adenosine 2'-adenylic acid, (2'-AMP), polyethylene glycol, X-ray diffraction, glycerol, glycosyl bond, C2'-endo ribose pucker, Tyr39, His41, Glu62, Arg85, His101, Asp29Oδ1, Val30O, Asn32Oδ1, Asp37Oδ
Abstract: Asparagine can be non-enzymatically deamidated and isomerized via succinimide to isoaspartate. This posttranslational modification can potentially alter the physical properties or the function of the parent protein. Asn32 of ribonuclease U2A from Ustilago sphaerogena is known to rapidly deamidate and isomerize in alkaline conditions. The crystal structure of ribonuclease U2A complexed with 2´-adenylic acid and calcium ions was determined at 1.03 Å resolution. In this structure, the region from Asp29 to Asp37 winds around a calcium ion, and the main-chain of Asn32 – Gly33 adopts an extended conformation. Rotation of the side-chain of Asn32 could bring Asn32Cγ into close proximity to Gly33N, in a conformation suitable for succinimide formation. The structure suggests that in solution the region around Asn32 – Gly33 is likely to be in equilibrium between multiple conformers, with the deamidation of Asn32 proceeding when the region adopts an extended conformation.
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Noguchi Shuji, Conformational Variation Revealed by the Crystal Structure of Rnase U2A Complexed with Ca Ion and 2´-Adenylic Acid at 1.03 Å Resolution, Protein & Peptide Letters 2010; 17 (12) . https://dx.doi.org/10.2174/0929866511009011559
DOI https://dx.doi.org/10.2174/0929866511009011559 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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