Abstract
Due to asymmetrical charge distribution of the mammalian high mobility group protein A2 (HMGA2), which makes HMGA2 bind to both cation- and anion-exchange columns, we developed a rapid procedure for purifying HMGA2 in the milligram range. This purification procedure greatly facilitated biophysical studies, which require large amounts of the protein.
Keywords: The mammalian high mobility group protein A2 (HMGA2), DNA binding protein, isothermal titration calorimetry, differential scanning calorimetry, nuclear magnetic resonance (NMR) Spectroscopy, intrinsically unstructured protein
Protein & Peptide Letters
Title: Large Scale Preparation of the Mammalian High Mobility Group Protein A2 for Biophysical Studies
Volume: 14 Issue: 1
Author(s): Tengjiao Cui, Suzanne Joynt, Victor Morillo, Maria Baez, Zhichun Hua, Xiaotang Wang and Fenfei Leng
Affiliation:
Keywords: The mammalian high mobility group protein A2 (HMGA2), DNA binding protein, isothermal titration calorimetry, differential scanning calorimetry, nuclear magnetic resonance (NMR) Spectroscopy, intrinsically unstructured protein
Abstract: Due to asymmetrical charge distribution of the mammalian high mobility group protein A2 (HMGA2), which makes HMGA2 bind to both cation- and anion-exchange columns, we developed a rapid procedure for purifying HMGA2 in the milligram range. This purification procedure greatly facilitated biophysical studies, which require large amounts of the protein.
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Cite this article as:
Cui Tengjiao, Joynt Suzanne, Morillo Victor, Baez Maria, Hua Zhichun, Wang Xiaotang and Leng Fenfei, Large Scale Preparation of the Mammalian High Mobility Group Protein A2 for Biophysical Studies, Protein & Peptide Letters 2007; 14 (1) . https://dx.doi.org/10.2174/092986607779117281
DOI https://dx.doi.org/10.2174/092986607779117281 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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