Abstract
The formation of amyloid fibrils are thought to contribute to pathogenesis of many amyloids associated human diseases. Here the impact of curcumin on amyloid formation of human serum albumin (HSA) was studied. Incubation of HSA at 68°C under physiologic pH led to amyloid fibril formation. Thioflavin T (ThT) fluorescence was used for determination of amyloid fibril formation. Atomic force microscopy experiments indicated different fibril structure of HSA incubated with or without curcumin. The monitoring of the changes in reactive oxygen species (ROS) levels upon incubation of curcumin with HSA showed a significant decrease in ROS levels. Similar experiments were also carried out in the presence of aflatoxin M1 (AFM1) and lead (Pb) ions. Our results indicated that AFM1 and Pb ions promote the fibrillation of HSA and accelerate ROS production, which were inhibited in the presence of curcumin. Thus, curcumin mitigates protein fibrillation activity and diminishes ROS generation.
Keywords: Aflatoxin M1, curcumin, fibrillation, HSA, lead ions, ROS.
Protein & Peptide Letters
Title:Curcumin Mitigates the Fibrillation of Human Serum Albumin and Diminishes the Formation of Reactive Oxygen Species
Volume: 22 Issue: 4
Author(s): Mansooreh Mazaheri, Ali Akbar Moosavi-Movahedi, Ali Akbar Saboury, Mehran Habibi Rezaei, Mostafa Shourian, Mohammad Farhadi and Nader Sheibani
Affiliation:
Keywords: Aflatoxin M1, curcumin, fibrillation, HSA, lead ions, ROS.
Abstract: The formation of amyloid fibrils are thought to contribute to pathogenesis of many amyloids associated human diseases. Here the impact of curcumin on amyloid formation of human serum albumin (HSA) was studied. Incubation of HSA at 68°C under physiologic pH led to amyloid fibril formation. Thioflavin T (ThT) fluorescence was used for determination of amyloid fibril formation. Atomic force microscopy experiments indicated different fibril structure of HSA incubated with or without curcumin. The monitoring of the changes in reactive oxygen species (ROS) levels upon incubation of curcumin with HSA showed a significant decrease in ROS levels. Similar experiments were also carried out in the presence of aflatoxin M1 (AFM1) and lead (Pb) ions. Our results indicated that AFM1 and Pb ions promote the fibrillation of HSA and accelerate ROS production, which were inhibited in the presence of curcumin. Thus, curcumin mitigates protein fibrillation activity and diminishes ROS generation.
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Mazaheri Mansooreh, Moosavi-Movahedi Akbar Ali, Saboury Akbar Ali, Rezaei Habibi Mehran, Shourian Mostafa, Farhadi Mohammad and Sheibani Nader, Curcumin Mitigates the Fibrillation of Human Serum Albumin and Diminishes the Formation of Reactive Oxygen Species, Protein & Peptide Letters 2015; 22 (4) . https://dx.doi.org/10.2174/0929866522666150209150004
DOI https://dx.doi.org/10.2174/0929866522666150209150004 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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