摘要
通过1-D凝胶电泳分析Pseudechis colletti和Pseudechis butleri毒液,然后进行从蛋白质条带获得的胰蛋白酶肽的质谱分析。两种毒液含有高度有效的药理活性成分,分配到以下蛋白质家族:碱性和酸性磷脂酶A2(PLA2s),L-氨基酸氧化酶(LAAO),P-III金属蛋白酶(P-III SVMP) 核苷酸(5''-NTDs),富含半胱氨酸的分泌蛋白(CRISP),毒液神经生长因子(VNGFs)和突触后神经毒素。PLA2s相对于其他毒素相当大的优势是两种毒液的特征。毒液组成中的主要差异是高血压和高血压的血清中的高浓度,以及突触后神经毒素的存在。此外,分析显示高浓度的蛋白质具有肌肉毒性,凝血功能和凋亡活性。PLA2s负责在患者术后观察到的毒性和抗凝血作用(4)。在两种毒液中遇到的其他蛋白质家族可能有助于这些毒液所描述的主要症状。这些结果解释了黑蛇病毒的观察临床效果。分析毒液含有具有医学意义的P-III金属蛋白酶,其用于血管性血友病因子(vWF)疾病的诊断目的,用于调节血栓形成和止血中的vWF,用于研究宿主防御中补体系统的功能 和疾病的发病机制。静脉数据的比较显示了来自Pseudechis蛇的主要毒液成分的相似性,导致了envenomation的常见临床效果,并证明了Elapidae蛇的毒液毒素之间的密切关系。
关键词: 蛋白质组,蛇毒,Pseudechis butleri,Pseudechis colletti,蛋白质谱分析。
Current Medicinal Chemistry
Title:Protein Profile Analysis of Two Australian Snake Venoms by One- Dimensional Gel Electrophoresis and MS/MS Experiments
Volume: 24 Issue: 17
关键词: 蛋白质组,蛇毒,Pseudechis butleri,Pseudechis colletti,蛋白质谱分析。
摘要: The Pseudechis colletti and Pseudechis butleri venoms were analyzed by 1-D gel electrophoresis, followed by mass spectrometric analysis of tryptic peptides obtained from the protein bands. Both venoms contain highly potent pharmacologically active components, which were assigned to the following protein families: basic and acidic phospholipases A2 (PLA2s), L-amino acid oxidases (LAAOs), P-III metalloproteinases (P-III SVMPs), 5’- nucleotidases (5’-NTDs), cysteine-rich secretory proteins (CRISPs), venom nerve growth factors (VNGFs) and post-synaptic neurotoxins. Considerable predominance of PLA2s over other toxins is a characteristic feature of both venoms. The major differences in the venom compositions are the higher concentration of SVMPs and CRISPs in the P. butleri venom, as well as the presence of post-synaptic neurotoxins. Furthermore, the analysis revealed a high concentration of proteins with myotoxic, coagulopathic and apoptotic activities. PLA2s are responsible for the myotoxic and anticoagulant effects observed in patients after envenomation (4). The other protein families, encountered in the two venoms, probably contribute to the major symptoms described for these venoms. These results explain the observed clinical effects of the black snake envenomation. The analyzed venoms contain group P-III metalloproteinases of medical importance with the potency to be used for diagnostic purposes of von Willebrand factor (vWF) disease, for regulation of vWF in thrombosis and haemostasis, for studying the function of the complement system in host defense and in the pathogenesis of diseases. Comparison of venomic data showed similarities in the major venom components of snakes from the genus Pseudechis, resulting in common clinical effects of envenomation, and demonstrating close relationships between venom toxins of Elapidae snakes.
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Protein Profile Analysis of Two Australian Snake Venoms by One- Dimensional Gel Electrophoresis and MS/MS Experiments, Current Medicinal Chemistry 2017; 24 (17) . https://dx.doi.org/10.2174/0929867324666170601073148
DOI https://dx.doi.org/10.2174/0929867324666170601073148 |
Print ISSN 0929-8673 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-533X |
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