摘要
miRNAs是非编码RNA分子,它们的反常调节可能导致癌症的发生。但是,miRNA功能紊乱是怎样导致弥漫性大B细胞淋巴瘤(DLBCL)的机制还没有很好的确认。在这项研究中,我们分析了在4个DLBCL细胞株和168个病人样本中miR-224的表达,我们发现miR-224的表达在DLBCL细胞株中与正常B细胞比较是下调的,但是在生发中心B细胞样和活化B细胞样之间在统计学上没有差异。利用生物信息学预测与荧光素酶报告化验分析,我们揭示了miR-224通过结合到CD59的3’端非翻译区的方式直接下调CD59表达。我们也使用免疫组化染色方式染色人DLBCL样本细胞的CD59,并分析miR-224,CD59的表达和分别使用利妥昔单抗,环磷酰胺,阿霉素,长春新碱和泼尼松(R-CHOP)治疗的DLBCL病人的总的/复发生存率的相关性。我们发现miR-224可能导致DLBCL的发生,更重要的是miR-224和CD59的表达能够预测R-CHOP治疗DLBCL病人后的反应和效果。
关键词: CD59,致弥漫性大B细胞淋巴瘤 (DLBCL),小分子RNA,miR-224, R-CHOP.
Current Cancer Drug Targets
Title:Deregulated Expression of miR-224 and its Target Gene: CD59 Predicts Outcome of Diffuse Large B-cell Lymphoma Patients Treated with R-CHOP
Volume: 14 Issue: 7
Author(s): Guoqi Song, Guorong Song, Huiyun Ni, Ling Gu, Hong Liu, Baoan Chen, Bangshun He, Yuqin Pan, Shukui Wang and William C. Cho
Affiliation:
关键词: CD59,致弥漫性大B细胞淋巴瘤 (DLBCL),小分子RNA,miR-224, R-CHOP.
摘要: miRNAs are non-coding RNA molecules; their deregulations may contribute to cancer pathogenesis. However, the mechanisms of how miRNA dysfunction contributes to the lymphomagenesis of diffuse large B-cell lymphoma (DLBCL) are not well established. In this study, we analyzed the expression of miR-224 in four DLBCL cell lines and 168 patients’ specimens. We found that the expression of miR-224 in DLBCL was down-regulated compared with normal B-cell but was not statistically different between the germinal center B-cell-like-type and the activated B-cell-like-type. Using bioinformatics prediction and luciferase report assays, we demonstrated that miR-224 directly down-regulated CD59 expression by binding to its 3’-untranslated region. We also used immunohistochemical staining of CD59 in human DLBCL specimens and analyzed the relationship between the expression of miR-224, CD59 and the overall/progress-free survival of DLBCL patients who were uniformly treated with rituximab,cyclophosphamide, adriamycin, vincristine, and prednisone (R-CHOP). We found that miR-224 may contribute to DLBCL pathogenesis. Most importantly, the expression of miR-224 and CD59 can predict the response and outcome of DLBCL patients treated with R-CHOP.
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Cite this article as:
Song Guoqi, Song Guorong, Ni Huiyun, Gu Ling, Liu Hong, Chen Baoan, He Bangshun, Pan Yuqin, Wang Shukui and Cho C. William, Deregulated Expression of miR-224 and its Target Gene: CD59 Predicts Outcome of Diffuse Large B-cell Lymphoma Patients Treated with R-CHOP, Current Cancer Drug Targets 2014; 14 (7) . https://dx.doi.org/10.2174/1568009614666140818211103
DOI https://dx.doi.org/10.2174/1568009614666140818211103 |
Print ISSN 1568-0096 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-5576 |
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