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Research Article

高葡萄糖影响培养的人间充质干细胞中雷帕霉素，二甲双胍和过氧化氢的细胞毒性潜力

卷 19, 期 9, 2019

页: [688 - 698] 页: 11

弟呕挨: 10.2174/1566524019666190722115842

价格: $65

摘要

背景：氧化应激和慢性高血糖症是2型糖尿病的两个主要副作用，影响所有类型的细胞，包括间充质干细胞（MSC）。 作为细胞疗法的选择，了解MSC的行为将为有效治疗提供关键信息。 方法：用各种浓度的葡萄糖，二甲双胍，雷帕霉素和过氧化氢处理胎盘间充质干细胞，以监测其活力和细胞周期分布。 通过MTT测定法检查细胞活力。 通过碘化丙锭染色研究细胞周期分布，并使用膜联蛋白V碘化丙锭染色和流式细胞术确定细胞凋亡。 通过蛋白质印迹评估Akt，P70S6K和AMPK的激活，评估潜在信号通路的参与程度。 结果：结果表明，高葡萄糖可增强细胞活力并降低二甲双胍的毒性潜力。 但是，过氧化氢和雷帕霉素的毒性加剧。 结论：我们的研究结果表明，在培养中存在雷帕霉素，二甲双胍和过氧化氢的情况下，高葡萄糖浓度对胎盘间充质干细胞的生存能力有重大影响。

关键词: 葡萄糖，雷帕霉素，二甲双胍，过氧化氢，间充质干细胞，细胞毒性潜能

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