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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Crystallization and Preliminary Crystallographic Analysis of Allograft Inflammatory Factor 1

Author(s): Shentao Li, Xiaoping Liao, Zhangliu Chen and Mark Bartlam

Volume 13, Issue 8, 2006

Page: [855 - 858] Pages: 4

DOI: 10.2174/092986606777841208

Price: $65


Allograft inflammatory factor-1 (AIF-1) is a 17-kDa IFN- γ inducible Ca2+-binding EF-hand protein involved in immune dysfunction and smooth muscle cell activation. AIF-1 was solubly expressed in E. coli and purified. Crystals of AIF-1 were grown at 291 K using PEG-8000 as precipitant. Diffraction by the AIF-1 crystal extends to 3.3 Å resolution, and the crystal belongs to the space group P43 with unit cell parameters a=b=73.4, c=49.1 Å .

Keywords: Allograft inflammatory factor 1, prokaryotic expression, crystallization

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