Crystallization and Preliminary Crystallographic Analysis of Allograft Inflammatory Factor 1

Title: Crystallization and Preliminary Crystallographic Analysis of Allograft Inflammatory Factor 1

Volume: 13 Issue: 8

Author(s): Shentao Li, Xiaoping Liao, Zhangliu Chen and Mark Bartlam

Affiliation:

Keywords: Allograft inflammatory factor 1, prokaryotic expression, crystallization

Abstract: Allograft inflammatory factor-1 (AIF-1) is a 17-kDa IFN- γ inducible Ca2+-binding EF-hand protein involved in immune dysfunction and smooth muscle cell activation. AIF-1 was solubly expressed in E. coli and purified. Crystals of AIF-1 were grown at 291 K using PEG-8000 as precipitant. Diffraction by the AIF-1 crystal extends to 3.3 Å resolution, and the crystal belongs to the space group P43 with unit cell parameters a=b=73.4, c=49.1 Å .

Cite this article as:

Li Shentao, Liao Xiaoping, Chen Zhangliu and Bartlam Mark, Crystallization and Preliminary Crystallographic Analysis of Allograft Inflammatory Factor 1, Protein & Peptide Letters 2006; 13(8) . https://dx.doi.org/10.2174/092986606777841208