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Recent Patents on Biotechnology


ISSN (Print): 1872-2083
ISSN (Online): 2212-4012

Research Article

Marine Algae of the Genus Gracilaria as Multi Products Source for Different Biotechnological and Medical Applications

Author(s): Asmaa Nabil-Adam*, Mohamed A. Shreadah, Nehad M. Abd El-Moneam and Samy A. El-Assar

Volume 14 , Issue 3 , 2020

Page: [203 - 228] Pages: 26

DOI: 10.2174/1872208314666200121144816

Price: $65


Background: Gracilaria has been shown to be an important source of marine bioactive natural biomaterials and compounds. Although there are no enough patents used Gracilaria worldwide, the current study tries to put the Gracilaria on the spot for further important patents in the future.

Objective: The current study investigates the pharmaceuticals and biochemical activity of Gracilaria because no previous studies have been carried out to examine the biochemical and pharmaceutical activates of Gracilaria from the Suez Canal of Egypt as an excellent source for bioactive compounds.

Methods: Different advanced experimental models and analytical techniques, such as cytotoxicity, total antioxidant capacity, anticancer, and anti-inflammatory profiling were applied. The phytochemical analysis of different constituents was also carried out.

Results: The mineral analysis revealed the presence of copper (188.3 ppm) and iron (10.07 ppm) in addition to a remarkable wealth of selenium and sulfur contents giving up to 36% of its dry mass. The elemental analysis showed high contents of sulfur and nitrogen compounds. The GCMS profiling showed varieties of different bioactive compounds, such as fatty acids, different types of carotenoids in addition to pigments, alkaloids, steroids. Many other compounds, such as carbohydrates and amino acids having antioxidant, anti-inflammatory, and antiviral activities, etc. were identified. The cytotoxicity activity of Gracilaria marine extract was very effective against cancerous cell lines and showed high ability as a potent antitumor due to their bioactive constituents. Specialized screening assays using two anticancer experimental models, i.e., PTK and SKH1 revealed 77.88% and 84.50% inhibition anticancer activity; respectively. The anti-inflammatory activities investigated using four different experimental models, i.e., COX1, COX2, IL6, and TNF resulted in 68%, 81.76%, 56.02% and 78.43% inhibition; respectively. Moreover, Gracilaria extracts showed potent anti-Alzheimer with all concentrations.

Conclusion: Gracilaria proved to be a multi-product source of marine natural products for different biotechnological applications. Our recommendation is to investigate the Gracilaria bioactive secondary metabolites in order to create and innovate in more patents from current important seaweeds (Gracilaria).

Keywords: Gracilaria genus, Suez Canal, biological activities, multi products source of marine bioactive natural compounds, biotechnological, applications, medical applications.

Graphical Abstract
Hegazy MEF, Mohamed TA, Elshamy AI, Hassanien AA, Abdel-Azim NS, Shreadah MA, et al. A new steroid from the Red Sea soft coral Lobophytum lobophytum. Nat Prod Res 2016; 30(3): 340-4.
[] [PMID: 26134487]
Hegazy MEF, Gamal-Eldeen AM, Mohamed TA, Alhammady MA, Hassanien AA, Shreadah MA, et al. New cytotoxic constituents from the red sea soft coral Nephthea sp. Nat Prod Res 2016; 30(11): 1266-72.
[] [PMID: 26165402]
Abdel Monein NM, Al-Assar SA, Shreadah MA, Nabil-Adam A. Isolation, identification and molecular screening of Pseudomonas sp. metabolic pathways nrps and pks associated with the red sea sponge, Hyrtios aff. Erectus, Egypt. J Pure Appl Microbiol 2017; 11(3): 1299-311.
Abdel Monein NM, Shreadah MA, Al-Assar SA, Nabil-Adam A. Protective role of antioxidants capacity of Hyrtios aff. Erectus sponge extract against mixture of persistent organic pollutants (POPs)-induced hepatic toxicity in mice liver: biomarkers and ultrastructural study. Environ Sci Pollut Res 2017; 24(27): 1-12.
Huang WY, Davidge ST, Wu J. Bioactive natural constituents from food sources-potential use in hypertension prevention and treatment. Crit Rev Food Sci Nutr 2013; 53(6): 615-30.
[] [PMID: 23627503]
Zhang R, Yuen AKL, Magnusson M, Wright JT, de Nys R, Masters AF, et al. A comparative assessment of the activity and structure of phlorotannins from the brown seaweed Carpophyllum flexuosum. Algal Res 2018; 29: 130.
de Almeida CL, Falcão Hde S, Lima GR, Montenegro CA, Lira NS, de Athayde-Filho PF, et al. Bioactivities from marine algae of the genus Gracilaria. Int J Mol Sci 2011; 12(7): 4550-73.
[] [PMID: 21845096]
Fernando PS, Sanjeewa KKA, Samarakoon KW, et al. FTIR characterization and antioxidant activity of water soluble crude polysaccharides of Sri Lankan marine algae. Algae 2017; 32(1): 75-86.
Ebrahimzadeh MA, Khalili M, Dehpour AA. Antioxidant activity of ethyl acetate and methanolic extracts of two marine algae, Nannochloropsis oculata and Gracilaria gracilis - an in vitro assay. Braz J Pharm Sci 2018; 54(1): 1-6.
Abo-el-Khair EM, Abdel-Fattah LM, Abdel-Halim AM, Aly-Eldeen M, Fahmy MA, Ahdy HH, et al. Assessment of the hydrochemical characteristics for the coastal waters of the Suez Gulf during 2011-2013. JEP 2016; 7: 1497-521.
Abdel Ghani SA, Shobier AH, Shreadah MA. Assessment of arsenic and vanadium pollution in surface sediments of the Egyptian Mediterranean Coast. J Environ Tech Manag 2013; 16(1/2): 82-101.
Said TO, Shreadah MA, Mansour MA, Mohamed MA, El-Sharkawi FM. OCPs, PCBs and THCs in Sparus auratus species from the Egyptian Mediterranean Coast. Open J Mar Sci 2017; 7(2): 317-26.
Masoud MS, Said TO, El-Zokm GM, Shreadah MA. Assessment of heavy metals contamination in surface sediments of the Egyptian Red Sea Coasts. AJBAS 2012; 6: 44-58.
El Zokm GM, Abdel Ghani SA, Shobier AH, Othman TS, Shreadah MA. IR spectroscopic investigation, X-ray structural characterization, thermal analysis decomposition and metal content of sediment samples along Egyptian Mediterranean Coast. WASJ 2013; 23(6): 823-36.
Annex VI - Environmental and Social Management Framework FP-UNDP-050617-5945- Annex VI (b) 2017.
Taga MS, Miller EE, Pratt DE. Chia seeds as a source of natural lipid antioxidants. J Am Oil Chem Soc 1984; 61(5): 928-31.
Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999; 64(4): 555-9.
Sun B, Richardo-Da-Silvia JM, Spranger I. Critical factors of vanillin assay for catechins and proanthocyanidins. J Agric Food Chem 1998; 46: 4267-74.
Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal 2006; 19(6-7): 669-75.
AOAC. Official Methods analysis of association of offcialanaltical chemists Washington DC, USA: association of official analytical chemists 1990.
Balaji D, Thamilvanan S, Vinayagam C, Balakumar BS. Anticancer, antioxidant activity and GC-MS analysis of selectedalgal members of Chlorophyceae. Int J Pharm Sci Rev Res 2017; 8(8): 3302-14.
Ellinger B, Silber J, Prashar A, Landskron J, Weber J, Rehermann S, et al. A phenotypic screening approach to identify anticancer compounds derived from marine fungi. Assay Drug Dev Technol 2014; 12(3): 162-75.
[] [PMID: 24735443]
Amarowicz R, Naczk M, Zadernowski R, Shahidi F. Antioxidant activity of condensed tannins of beach pea, Canola hulls, evening primrose, and faba bean. J Food Lipids 2000; 7: 195-205.
Chakraborty K, Lipton AP, Paul Raj R, Vijayan KK. Antibacterial labdane diterpenoids of Ulva fasciata Delile from southwestern coast of the Indian Peninsula. Food Chem 2010; 119: 1399-408.
Moyo SJ, Aboud S, Kasubi M, Lyamuya EF, Maselle SY. Antimicrobial resistance among producers and non-producers of extended spectrum beta-lactamases in urinary isolates at a tertiary Hospital in Tanzania. BMC Res Notes 2010; 3: 348.
[] [PMID: 21184671]
Fonteh PN, Keter FK, Meyer D. New bis(thiosemicarbazonate) gold(III) complexes inhibit HIV replication at cytostatic concentrations: potential for incorporation into virostatic cocktails. J Inorg Biochem 2011; 105(9): 1173-80.
[] [PMID: 21708102]
Tabarsa M, Rezaei M, Ramezanpour Z, Waaland JR. Chemical compositions of the marine algae Gracilaria salicornia (Rhodophyta) and Ulva lactuca (Chlorophyta) as a potential food source. J Sci Food Agric 2012; 92(12): 2500-6.
[] [PMID: 22467477]
Ruperez P. Mineral content of edible marine seaweeds. Food Chem 2002; 79: 23-6.
Souza BWS, Cerqueira MA, Pinheiro AA, Pinheiroa AC, Martins JT, Teixeira JA, et al. Chemical characterization and antioxidant activity of sulfated polysaccharide from the red seaweed Gracilaria birdiae. Food Hydrocoll 2012; 27(2): 287-92.
Abirami RG, Kowsalya S. Phytochemical screening, microbial load and antimicrobial activity of underexploited seaweeds. Int Res J Microbiol 2012; 3(10): 328-32.
Oranday MA, Verde MSJ, Martínez-Lozano NH, Waksman J. Active fractions from four species of marine algae. Phyton 2004; 73: 165-70.
Cardozo KHM, Guaratini T, Barros MP, Falcão VR, Tonon AP, Lopes NP, et al. Metabolites from algae with economical impact. Comp Biochem Physiol C Toxicol Pharmacol 2007; 146(1-2): 60-78.
[] [PMID: 16901759]
Cen-Pacheco F, Nordström L, Souto ML, Martín MN, Fernández JJ, Daranas AH. Studies on polyethers produced by red algae. Mar Drugs 2010; 8(4): 1178-88.
[] [PMID: 20479973]
Chakraborty K, Joseph D, Praveen NK. Antioxidant activities and phenolic contents of three red seaweeds (Division: Rhodophyta) harvested from the Gulf of Mannar of Peninsular India. J Food Sci Technol 2015; 52(4): 1924-35.
[] [PMID: 25829573]
Ghannadi A, Shabani L, Yegdaneh A. Cytotoxic, antioxidant and phytochemical analysis of Gracilaria species from Persian Gulf. Adv Biomed Res 2016; 30(5): 139.
O’Sullivan L, Murphy B, McLoughlin P, Duggan P, Lawlor PG, Hughes H, et al. Prebiotics from marine macroalgae for human and animal health applications. Mar Drugs 2010; 8(7): 2038-64.
[] [PMID: 20714423]
Thenmozhi AJ, Manivasagam T, Essa MM. Role of plant polyphenols in Alzheimer’s disease. Adv Neurobiol 2016; 12: 153-71.
[] [PMID: 27651253]
Shreadah MA, Moneam NMAE, El-Assar SA, Nabil-Adam A. Metabolomics and pharmacological screening of Aspergillus versicolor isolated from Hyrtios erectus Red Sea Sponge: Egypt. Curr Bioact Compd. 2019.[Epub ahead of print].
[ 711]
Jouanne M, Rault S, Voisin-Chiret AS. Tau protein aggregation in Alzheimer’s disease: an attractive target for the development of novel therapeutic agents. Eur J Med Chem 2017; 139: 153-67.
[] [PMID: 28800454]
Nita M, Grzybowski A. The role of the reactive oxygen species and oxidative stress in the pathomechanism of the age-related ocular diseases and other pathologies of the anterior and posterior eye segments in adults. Oxid Med Cell Longev 2016. Epub ahead of print
Guo J, Zhou YJ, Hillwig ML, Shen Y, Yang L, Wang Y, et al. CYP76AH1 catalyzes turnover of miltiradiene in tanshinones biosynthesis and enables heterologous production of ferruginol in yeasts. Proc Natl Acad Sci USA 2013; 110(29): 12108-13.
[] [PMID: 23812755]
Khan MH, Salomaa SI, Jacquemet G, Butt U, Miihkinen M, Deguchi T, et al. The Sharpin interactome reveals a role for Sharpin in lamellipodium formation via the Arp2/3 complex. J Cell Sci 2017; 130(18): 3094-107.
[] [PMID: 28775156]
Joshi AS, Fei N, Greenberg ML. Get1p and Get2p are required for maintenance of mitochondrial morphology and normal cardiolipin levels. FEMS Yeast Res 2016; 16(3): 2016.
[] [PMID: 26926495]
Gammone MA, Riccioni G, D’Orazio N. Marine Carotenoids against oxidative stress: effects on human health. Mar Drugs 2015; 13(10): 6226-46.
[] [PMID: 26437420]
D’Orazio N, Gammone MA, Gemello E, De Girolamo M, Cusenza S, Riccioni G. Marine bioactives: pharmacological properties and potential applications against inflammatory diseases. Mar Drugs 2012; 10(4): 812-33.
[] [PMID: 22690145]
Ahmadi A, Zorofchian MS, Abubakar S, Zandi K. Antiviral potential of algae polysaccharides isolated from marine sources: a review. BioMed Res Int 2015; 2015: 825203.
[] [PMID: 26484353]
Yasuhara-Bell J, Lu Y. Marine compounds and their antiviral activities. Antiviral Res 2010; 86(3): 231-40.
[] [PMID: 20338196]
Noda F, Noda M, Nakamura T, Yoshino I, Miyamoto K, Yamada K, et al. Method of producing seaweed and seaweed paste obtained by such a method. US Patent OO28899A1 2009.
Murano E, Toffanin R, Cecere E, Rizzo R, Knutsen SR. Investigation of the carrageenans extracted from Solieria filiformis and Agrdhiella subulata from Mar Piccolo, Taranto. Mar Chem 1997; 58: 319-25.
[ (97)00058-3.]
Ohishi H, Hattori T, Tani H, Horiuchi S. Seaweedderived immunostimulant and antiinflammatory agent WO Patent 059819A1 2008.
Alan DB. Seaweed extract composition for treatment of inflammation US Patent 0050196410A1 2005.

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