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Current Analytical Chemistry


ISSN (Print): 1573-4110
ISSN (Online): 1875-6727

Chlorella Modified Glassy Carbon Electrode as Whole Cell Microbial Sensor for Heavy Metal Ions

Author(s): Susheel K. Mittal, J. Singh and Ashok Kumar SK

Volume 8 , Issue 3 , 2012

Page: [365 - 372] Pages: 8

DOI: 10.2174/157341112801264932

Price: $65


A whole cell based biosensor is prepared by immobilizing Chlorella sp. microbes over glassy carbon electrode. The proposed biosensor is optimized for characteristics like; substrate concentration, pH, response time, durability, etc. The electrode responds linearly in concentration range of 10-14 M to 10-6 M for zinc, copper, cadmium, cobalt and nickel ions. Relative selectivity of the metal ions can be ordered in following sequence: Zn2+ >> Cu2+ ≈ Cd2+ > Co2+ > Ni2+. The biosensor has a life time of 14 days, after which, its analytical properties do not remain stable.

Keywords: Atomic absorbtion spectrometry, biosensor, bovine serum albumin, Chlorella sp., chronoamperometry, glutaraldehyde, glassy carbon electrode, heavy metal ions, immobilization, interference, real life samples, ultra- trace level measurement

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