Abstract
A whole cell based biosensor is prepared by immobilizing Chlorella sp. microbes over glassy carbon electrode. The proposed biosensor is optimized for characteristics like; substrate concentration, pH, response time, durability, etc. The electrode responds linearly in concentration range of 10-14 M to 10-6 M for zinc, copper, cadmium, cobalt and nickel ions. Relative selectivity of the metal ions can be ordered in following sequence: Zn2+ >> Cu2+ ≈ Cd2+ > Co2+ > Ni2+. The biosensor has a life time of 14 days, after which, its analytical properties do not remain stable.
Keywords: Atomic absorbtion spectrometry, biosensor, bovine serum albumin, Chlorella sp., chronoamperometry, glutaraldehyde, glassy carbon electrode, heavy metal ions, immobilization, interference, real life samples, ultra- trace level measurement
Current Analytical Chemistry
Title:Chlorella Modified Glassy Carbon Electrode as Whole Cell Microbial Sensor for Heavy Metal Ions
Volume: 8 Issue: 3
Author(s): Susheel K. Mittal, J. Singh and Ashok Kumar SK
Affiliation:
Keywords: Atomic absorbtion spectrometry, biosensor, bovine serum albumin, Chlorella sp., chronoamperometry, glutaraldehyde, glassy carbon electrode, heavy metal ions, immobilization, interference, real life samples, ultra- trace level measurement
Abstract: A whole cell based biosensor is prepared by immobilizing Chlorella sp. microbes over glassy carbon electrode. The proposed biosensor is optimized for characteristics like; substrate concentration, pH, response time, durability, etc. The electrode responds linearly in concentration range of 10-14 M to 10-6 M for zinc, copper, cadmium, cobalt and nickel ions. Relative selectivity of the metal ions can be ordered in following sequence: Zn2+ >> Cu2+ ≈ Cd2+ > Co2+ > Ni2+. The biosensor has a life time of 14 days, after which, its analytical properties do not remain stable.
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Cite this article as:
K. Mittal Susheel, Singh J. and Kumar SK Ashok, Chlorella Modified Glassy Carbon Electrode as Whole Cell Microbial Sensor for Heavy Metal Ions, Current Analytical Chemistry 2012; 8 (3) . https://dx.doi.org/10.2174/157341112801264932
DOI https://dx.doi.org/10.2174/157341112801264932 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
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