Using Electrospray Ionisation Mass Spectrometry to Study Non-Covalent Interactions

Author(s): Bryan J. McCullough, Simon J. Gaskell.

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 12 , Issue 2 , 2009

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Abstract:

The key strengths of electrospray over any other ionisation techniques are its soft nature and its ability to produce multiply charged ions. This combination is ideal for the study of non-covalent interactions. In this review article, we cover the basics of studying non-covalent interactions by mass spectrometry - illustrated with examples from our own and other labs - and discuss the current mass spectrometry based methods used for understanding and characterising noncovalent protein complexes.

Keywords: Mass Spectrometry, Electrospray Ionisation, Non-Covalent Interactions, ESI mass spectrum, PROTEIN-LIGAND INTERACTIONS

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Article Details

VOLUME: 12
ISSUE: 2
Year: 2009
Page: [203 - 211]
Pages: 9
DOI: 10.2174/138620709787315463
Price: $65

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