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Current Bioactive Compounds

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ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

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Research Article

Preliminary Phytochemical Screening, Evaluation of the Phenolic Compositions and Antioxidant Activities of Four Iranian Alyssum Species

Author(s): Pooran Golkar*, Ahmad Fotoohi and Claudio Frezza

Volume 16, Issue 5, 2020

Page: [581 - 587] Pages: 7

DOI: 10.2174/1573407215666190215152137

Price: $65

Abstract

Background: Alyssum L. is a genus of herbaceous perennial or annual plants belonging to the Brassicaceae family. Little is known about biochemical properties of Alyssum species, specially endemics to Iran. In particular, the species were A. homolocarpum (Fisch. & C.A.Mey.) Boiss., A. lepidotum Boiss., A. maritimum (L.) Lam. (now syn. of Lobularia maritima (L.) Desv.) and A. simplex Rudolph. and eight accessions were studied. These diverse species accessions were collected in six different areas.

Materials and Methods: In this work, the Total Phenolic Content (TPC), the Total Flavonoid Content (TFD), the Total Flavonol Content (TFL), the Carotenoid content (Car) and the Anthocyanin content (Ant) of the leaf methanolic extracts of four Alyssum L. species collected in Iran, were assessed. The antioxidant activity assay for every extract obtained from the eight accessions was also carried out according to three distinct methodologies including three different methods including: DPPH, Beta carotene/ linoleic acid and phosphomolibdate assays.

Results: The highest TPC, TFD and TFL values were separately observed in two different accessions of A. lepidotum whereas the highest carotenoid content was observed in one accession of A. homolocarpum and the highest anthocyanin content was observed in A. maritimum. Different results were observed for different methodolies for antioxidant evaluations methods and some of them were found to have values, expressed in mg/mL, much lower with respect to the control. The least DPPH activity and the highest total antioxidant activity with phosphomolybdate assay was found in A. lepidotum.

Conclusion: The preliminary phytochemical screening and the evaluation of their antioxidant activities were reported here for the first time for Iranian Alyssum species. The findings eventually recommends, the use of those accessions in the ethnopharmacological and nutraceutical fields.

Keywords: antioxidant activities, ethnopharmacology and nutraceutics, Iranian Alyssum L. spp., leaf methanolic extracts, phytochemical analysis.

Graphical Abstract

[1]
Pignatti, S. Flora d’Italia; Edagricole: Bologna, 1982.
[2]
Bibi, T.; Ahmad, M.; Bakhsh Tareen, R.; Mohammad Tareen, N.; Jabeen, R.; Rehman, S.U.; Sultana, S.; Zafar, M.; Yaseen, G. Ethnobotany of medicinal plants in district Mastung of Balochistan province-Pakistan. J. Ethnopharmacol., 2014, 157, 79-89.
[http://dx.doi.org/10.1016/j.jep.2014.08.042] [PMID: 25260579]
[3]
Sadeghi, Z.; Kuhestani, K.; Abdollahi, V.; Mahmood, A. Ethnopharmacological studies of indigenous medicinal plants of Saravan region, Baluchistan, Iran. J. Ethnopharmacol., 2014, 153(1), 111-118.
[http://dx.doi.org/10.1016/j.jep.2014.01.007] [PMID: 24509152]
[4]
Hedge, J.; Rechinger, K.H. Cruciferae. Flora Iranica; Rechinger, K.H. Ed.; Akademische; Druck-u Verlagsanstalt: Graz, Austria, 1968, pp. 1-372.
[5]
Bahmani, M.; Zargaran, A. Ethno-botanical medicines used for urinary stones in the Urmia, Northwest Iran. Eur. J. Integr. Med., 2015, 7(6), 657-660.
[http://dx.doi.org/10.1016/j.eujim.2015.09.006]
[6]
Souri, E.; Amin, G.; Farsam, H.; Tehrani, M.B. Screening of antioxidant activity and phenolic content of 24 medicinal plant extracts. Daru, 2008, 16, 83-87.
[7]
Ozay, C.; Mammadov, R. Assessment of some biological activities of Alyssum L. known as madwort. Acta Pol. Pharm., 2016, 73(5), 1213-1220.
[PMID: 29638061]
[8]
Hamedi, A.; Ghanbari, A.; Razavipour, R.; Saeidi, V.; Zarshenas, M.M.; Sohrabpour, M.; Azari, H. Alyssum homolocarpum seeds: phytochemical analysis and effects of the seed oil on neural stem cell proliferation and differentiation. J. Nat. Med., 2015, 69(3), 387-396.
[http://dx.doi.org/10.1007/s11418-015-0905-9] [PMID: 25860174]
[9]
Maghsoudlu, M.T.; Valizade, J.; Ebrahimianchavoshlou, S.; Mohammadi Bolbolanabad, M.; Rahneshan, N. Phytochemcial and antioxidant activity of Alyssum maritimum and Achillea wilhelmsii from Sistan and Baluchestan province. Eco-phytochem. J. Med. Pl., 2014, 2(3), 1-9.
[10]
Afsharypuor, S.; Lockwood, G.B. Flavonoid Glucuronides from Alyssum minimum. J. Nat. Prod., 1986, 49(5), 944-945.
[http://dx.doi.org/10.1021/np50047a041]
[11]
Ozay, C.; Mammadov, R. Screening of some biological activities of Alyssum fulvescens var. fulvescens known as ege madwort. Acta Biol. Hung., 2017, 68(3), 310-320.
[http://dx.doi.org/10.1556/018.68.2017.3.8] [PMID: 28901801]
[12]
Miliauskas, G.; Venskutonis, P.; Van Beek, T. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem., 2004, 85, 231-237.
[http://dx.doi.org/10.1016/j.foodchem.2003.05.007]
[13]
Gharibi, S.; Tabatabaei, B.E.S.; Saeidi, G. Comparison of essential oil composition, flavonoid content and antioxidant activity in eight Achillea species. J. Essent. Oil Bear. Pl., 2015, 18, 1382-1394.
[http://dx.doi.org/10.1080/0972060X.2014.981600]
[14]
Souri, M.; Shakeri, A. Optimization of total phenol and tannin content and biological activity of Dittrichia graveolens (L.) GREUTER. Curr. Bioact. Compd., 2018, 14, 1.
[15]
Salem, N.; Msaada, K.; Hamdaoui, G.; Limam, F.; Marzouk, B. Variation in phenolic composition and antioxidant activity during flower development of safflower (Carthamus tinctorius L.). J. Agric. Food Chem., 2011, 59(9), 4455-4463.
[http://dx.doi.org/10.1021/jf1049936] [PMID: 21434653]
[16]
Falcone Ferreyra, M.L.; Rius, S.P.; Casati, P. Flavonoids: biosynthesis, biological functions, and biotechnological applications. Front. Plant Sci., 2012, 3, 222.
[http://dx.doi.org/10.3389/fpls.2012.00222] [PMID: 23060891]
[17]
Dewick, P.M. Chimica, Biosintesi e Bioattività delle Sostanze Naturali Piccin, Ed.;
[18]
Akkol, E.K.; Göger, F.; Koşar, M.; Başer, K.H.C. Phenolic composition and biological activities of Salvia halophila and Salvia virgata from Turkey. Food Chem., 2008, 108(3), 942-949.
[http://dx.doi.org/10.1016/j.foodchem.2007.11.071] [PMID: 26065756]
[19]
Kumar, P.P.; Kumaravel, S.; Lalitha, C. Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo. Afr. J. Biochem. Res., 2010, 4, 191-195.
[20]
Pollastri, S.; Tattini, M. Flavonols: old compounds for old roles. Ann. Bot., 2011, 108(7), 1225-1233.
[http://dx.doi.org/10.1093/aob/mcr234] [PMID: 21880658]
[21]
Bouzidi, S.; Benkiki, N.; Hachemi, M.; Haba, H. Investigation of in vitro antioxidant activity and in vivo antipyretic and anti-inflammatory activities of Algerian Eryngium campestre L. Curr. Bioact. Compd., 2017, 13(4), 340-346.
[http://dx.doi.org/10.2174/1573407212666160815124204]
[22]
Koleva, I.I.; van Beek, T.A.; Linssen, J.P.; de Groot, A.; Evstatieva, L.N. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem. Anal., 2002, 13(1), 8-17.
[http://dx.doi.org/10.1002/pca.611] [PMID: 11899609]
[23]
Abootalebian, M.; Keramat, J.; Kadivar, M.; Ahmadi, F.; Abdinian, M. Comparison of total phenolic and antioxidant activity of different Mentha spicata and M. longifolia accessions. Ann. Agric. Sci., 2016, 61, 175-179.
[http://dx.doi.org/10.1016/j.aoas.2016.10.002]
[24]
Lichtenthaler, H.K.; Wellburn, A.R. Determinations of total carotenoids and chlorophylls A and B of leaf extracts in different solvents. Biochem. Soc. Trans., 1983, 11, 591-592.
[http://dx.doi.org/10.1042/bst0110591]
[25]
Luis, A.; Gil, C.; Duarte, A.P. Antioxidant activity of extracts of Portuguese shrubs: Pterospartum tridentatum, Cytisus scoparius and Erica spp. J. Med. Plants Res., 2009, 3, 886-893.
[26]
Yamamoto, Y.; Gaynor, R.B. Therapeutic potential of inhibition of the NF-kappaB pathway in the treatment of inflammation and cancer. J. Clin. Invest., 2001, 107(2), 135-142.
[http://dx.doi.org/10.1172/JCI11914] [PMID: 11160126]
[27]
Cazarolli, L.H.; Zanatta, L.; Alberton, E.H.; Figueiredo, M.S.; Folador, P.; Damazio, R.G.; Pizzolatti, M.G.; Silva, F.R. Flavonoids: prospective drug candidates. Mini Rev. Med. Chem., 2008, 8(13), 1429-1440.
[http://dx.doi.org/10.2174/138955708786369564] [PMID: 18991758]
[28]
Cushnie, T.P.; Lamb, A.J. Antimicrobial activity of flavonoids. Int. J. Antimicrob. Agents, 2005, 26(5), 343-356.
[http://dx.doi.org/10.1016/j.ijantimicag.2005.09.002] [PMID: 16323269]
[29]
Schroeder, J. Pharmacopoeia medico-chymica: Sive thesaurus pharmacologeus. Ulmae Suevorum: Johannis Gerlini, 1655.
[30]
Berends, K.A.W. Handbuch der praktischen Arzneiwissenschaft oder der speziellen Pathologie und Therapie; Enslin: Berlin, 1829.
[31]
He, F.; Mu, L.; Yan, G.L.; Liang, N.N.; Pan, Q.H.; Wang, J.; Reeves, M.J.; Duan, C.Q. Biosynthesis of anthocyanins and their regulation in colored grapes. Molecules, 2010, 15(12), 9057-9091.
[http://dx.doi.org/10.3390/molecules15129057] [PMID: 21150825]
[32]
Leoncini, E.; Nedovic, D.; Panic, N.; Pastorino, R.; Edefonti, V.; Boccia, S. Carotenoid intake from natural sources and head and neck cancer: A systematic review and meta-analysis of epidemiological studies. Cancer Epidemiol. Biomarkers Prev., 2015, 24(7), 1003-1011.
[http://dx.doi.org/10.1158/1055-9965.EPI-15-0053] [PMID: 25873578]
[33]
Hara, M.; Oki, K.; Hoshino, K.; Kuboi, T. Enhancement of anthocyanin biosynthesis by sugar in radish (Raphanus sativus) hypocotyl. Plant Sci., 2003, 164, 259-265.
[http://dx.doi.org/10.1016/S0168-9452(02)00408-9]
[34]
Keffous, F.; Belboukhari, N.; Djaradi, H.; Cheriti, A.; Sekkoum, K.; Aboul-Enein, H.Y. Total antioxidant capacity, reducing power and cyclic voltammetry of Zilla Macroptera (Brassicaceae) Aqueous extract. Curr. Bioact. Compd., 2016, 12(1), 39-43.
[http://dx.doi.org/10.2174/1573407212666160210230707]
[35]
SAS Institute SAS/STAT User’s Guide, Version 9. 3; SAS Institute: Cary, NC, 2011.

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