Abstract
DNA polymerase I enzymes have served as model systems to study the mechanism of template-directed DNA polymerization. This process requires that the enzyme cycles through a series of conformational changes, each cycle leading to the incorporation of a nucleotide to the primer strand of the DNA. The kinetics of nucleotide incorporation has been extensively studied leading to the definition of specific steps along the cycle. Efforts to visualize these steps using X-ray crystallography have recently come to fruition, notably for one particular DNA polymerase I system, that of Klentaq1. This review focuses on the structural characterization of the various steps along the nucleotide incorporation pathway.
Keywords: Klentaq1 DNA Polymerase, Thermus aquaticus, DNTP, deoxynucleoside-5-triphosphate, 5-fluorosulfonyladenosine, NUCLEOTIDYL TRANSFER, REVERSE TRANSITION, PYROPHOSPHATE, Two-metal-ion, TRANSLOCATION, NUCLEOTIDE SELECTIVITY
Current Organic Chemistry
Title: Structural Studies of the Klentaq1 DNA Polymerase
Volume: 5 Issue: 8
Author(s): Ying Li and Gabriel Waksman
Affiliation:
Keywords: Klentaq1 DNA Polymerase, Thermus aquaticus, DNTP, deoxynucleoside-5-triphosphate, 5-fluorosulfonyladenosine, NUCLEOTIDYL TRANSFER, REVERSE TRANSITION, PYROPHOSPHATE, Two-metal-ion, TRANSLOCATION, NUCLEOTIDE SELECTIVITY
Abstract: DNA polymerase I enzymes have served as model systems to study the mechanism of template-directed DNA polymerization. This process requires that the enzyme cycles through a series of conformational changes, each cycle leading to the incorporation of a nucleotide to the primer strand of the DNA. The kinetics of nucleotide incorporation has been extensively studied leading to the definition of specific steps along the cycle. Efforts to visualize these steps using X-ray crystallography have recently come to fruition, notably for one particular DNA polymerase I system, that of Klentaq1. This review focuses on the structural characterization of the various steps along the nucleotide incorporation pathway.
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Cite this article as:
Li Ying and Waksman Gabriel, Structural Studies of the Klentaq1 DNA Polymerase, Current Organic Chemistry 2001; 5 (8) . https://dx.doi.org/10.2174/1385272013375067
DOI https://dx.doi.org/10.2174/1385272013375067 |
Print ISSN 1385-2728 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5348 |
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