Background: Protein-Protein interaction (PPI) network analysis of virulence proteins of Aspergillus
fumigatus is a prevailing strategy to understand the mechanism behind the virulence of A. fumigatus.
The identification of major hub proteins and targeting the hub protein as a new antifungal drug
target will help in treating the invasive aspergillosis.
Materials & Method: In the present study, the PPI network of 96 virulence (drug target) proteins of A.
fumigatus were investigated which resulted in 103 nodes and 430 edges. Topological enrichment analysis
of the PPI network was also carried out by using STRING database and Network analyzer a cytoscape
plugin app. The key enriched KEGG pathway and protein domains were analyzed by STRING.
Conclusion: Manual curation of PPI data identified three proteins (PyrABCN-43, AroM-34, and Glt1-
34) of A. fumigatus possessing the highest interacting partners. Top 10% hub proteins were also identified
from the network using cytohubba on the basis of seven algorithms, i.e. betweenness, radiality,
closeness, degree, bottleneck, MCC and EPC. Homology model and the active pocket of top three hub
proteins were also predicted.