Abstract
The great potential of human embryonic and adult stem cells in regenerative medicine, gene therapy, drug discovery, and basic research is widely recognized. Many future applications depend on our ability to manipulate stem cell genomes with exogenous DNA in a safe and controllable way. Foreign DNA which carries, for example, a therapeutically active gene, a key regulatory gene involved in cell differentiation, or encodes for small interfering RNA may be inserted into or excised from stem cell genomes. Here, we review current progress and technical challenges in this field of genetic modification with a focus on controlled gene insertion into and ablation from stem cell genomes mediated by site-specific or homologous DNA recombination.
Keywords: Genome engineering, human stem cells, insertional mutagenesis, genome stability, gene targeting
Current Genomics
Title: High-Precision Genome Surgery in Human Stem Cells
Volume: 7 Issue: 7
Author(s): Ruijie Zhou and Peter Droge
Affiliation:
Keywords: Genome engineering, human stem cells, insertional mutagenesis, genome stability, gene targeting
Abstract: The great potential of human embryonic and adult stem cells in regenerative medicine, gene therapy, drug discovery, and basic research is widely recognized. Many future applications depend on our ability to manipulate stem cell genomes with exogenous DNA in a safe and controllable way. Foreign DNA which carries, for example, a therapeutically active gene, a key regulatory gene involved in cell differentiation, or encodes for small interfering RNA may be inserted into or excised from stem cell genomes. Here, we review current progress and technical challenges in this field of genetic modification with a focus on controlled gene insertion into and ablation from stem cell genomes mediated by site-specific or homologous DNA recombination.
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Cite this article as:
Zhou Ruijie and Droge Peter, High-Precision Genome Surgery in Human Stem Cells, Current Genomics 2006; 7 (7) . https://dx.doi.org/10.2174/138920206779116774
DOI https://dx.doi.org/10.2174/138920206779116774 |
Print ISSN 1389-2029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5488 |
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