The analytical bias introduced by most of the commonly used techniques in metabolomics
considerably hinders the simultaneous detection of all metabolites present in complex biological
samples. In order to solve this limitation, the combination of complementary approaches is emerging
in recent years as the most suitable strategy in order to maximize metabolite coverage. This review
article presents a general overview of the most important analytical techniques usually employed in
metabolomics: nuclear magnetic resonance, mass spectrometry and hybrid approaches. Furthermore,
we emphasize the potential of integrating various tools in the form of metabolomic multi-platforms in
order to get a deeper metabolome characterization, for which a revision of the existing literature in
this field is provided. This review is not intended to be exhaustive but, rather, to give a practical and
concise guide to readers not familiar with analytical chemistry on the considerations to account for
the proper selection of the technique to be used in a metabolomic experiment in biomedical research.
Keywords: Metabolomics, Multi-platform, Mass spectrometry, Nuclear magnetic resonance, Combination, Biomedical research.
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