Background: The positional effects of the methoxy- and hydroxyl substituents in the phenyl
ring were examined in vivo for distinct receptor classes in order to gain an insight into the mechanism
by which isomeric compounds (2S,4R,4aR,8R,8aR)-2-(3(4)-hydroxy-4(3)-methoxyphenyl)-4,7-dimethyl-
3,4,4a,5,8, 8a-hexahydro-2H-chromene-4,8-diols 1 and 2 exhibit their pharmacological activity.
Conclusion: Our findings suggest a strong structure-function relationship between the substitution pattern
and the mechanism of biological activity of compound. The methoxy substituent at C4 and the hydroxyl
substituent at C3 (compound 1) seem to employ the cannabinoid and adrenergic systems, whereas
compound 2 with the methoxy substituent at C3 and the hydroxyl substituent at C4 possibly targets the
opioid and dopaminergic mechanisms.