Protective effects of andrographolide derivative AL-1 on high glucose-induced oxidative stress in RIN-m cells

Author(s): Hui Yan, Yongmei Li, Yali Yang, Zaijun Zhang, Gaoxiao Zhang, Yewei Sun, Pei Yu, Yuqiang Wang, Lipeng Xu.

Journal Name: Current Pharmaceutical Design

Volume 22 , Issue 4 , 2016

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Abstract:

AL-1 is a novel andrographolide derivative synthesized by conjugating andrographolide and alpha lipoic acid. AL-1 has been found to increase insulin secretion, decrease blood glucose level and protect β-cell mass and function in alloxan-induced diabetic mouse model. However, the protective mechanism of AL-1 on high glucose-induced pancreatic β-cell injury is still not clear. In the present study, we found that AL-1 reduced reactive oxygen species (ROS) and nitric oxide (NO) generation induced by high glucose in RIN-m cells, and which elevated the activities of superoxide dismutase (SOD) and catalase (CAT). In addition, AL-1 increased the expression of NF-E2-related factor 2 (Nrf2), thioredoxin-1 (Trx-1) and heme oxygenase-1 (HO- 1) proteins in RIN-m cells. These results suggest that AL-1 prevented RIN-m cells from high glucose-induced oxidative damage via upregulation of Nrf2 signaling pathway.

Keywords: Andrographolide, α-Lipoic acid, Nrf2, ROS.

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Article Details

VOLUME: 22
ISSUE: 4
Year: 2016
Page: [499 - 505]
Pages: 7
DOI: 10.2174/1381612821666150921110716
Price: $58

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