Artificial skeletal muscle tissues composed of cells are expected to be used for applications of regenerative
medicine and drug screening. Generally, however, the physical forces generated by tissue-engineered skeletal muscle are
lower than those of skeletal muscle tissues found in the body. Local hyperthermia is used for many diseases including
muscle injuries. It was recently reported that mild heat treatment improved skeletal muscle functions. In this study, we investigated
the effects of mild heat treatment on the tissue-engineered skeletal muscle tissues in vitro. We used magnetite
cationic liposomes to label C2C12 myoblast cells magnetically, and constructed densely packed artificial skeletal muscle
tissues by using magnetic force. Cell culture at 39°C promoted the differentiation of myoblast cells into myotubes.
Moreover, the mild and transient heat treatment improved the contractile properties of artificial skeletal muscle tissue constructs.
These findings indicate that the culture method using heat treatment is a useful approach to enhance functions of
artificial skeletal muscle tissue.
Keywords: C2C12 cells, contractile force, heat treatment, magnetic force, skeletal muscle, tissue engineering.
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