Enhancement of Contractile Force Generation of Artificial Skeletal Muscle Tissues by Mild and Transient Heat Treatment

Author(s): Masanori Sato , Kazushi Ikeda , Shota Kanno , Akira Ito , Yoshinori Kawabe , Masamichi Kamihira .

Journal Name: Current Pharmaceutical Biotechnology

Volume 14 , Issue 13 , 2013

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Abstract:

Artificial skeletal muscle tissues composed of cells are expected to be used for applications of regenerative medicine and drug screening. Generally, however, the physical forces generated by tissue-engineered skeletal muscle are lower than those of skeletal muscle tissues found in the body. Local hyperthermia is used for many diseases including muscle injuries. It was recently reported that mild heat treatment improved skeletal muscle functions. In this study, we investigated the effects of mild heat treatment on the tissue-engineered skeletal muscle tissues in vitro. We used magnetite cationic liposomes to label C2C12 myoblast cells magnetically, and constructed densely packed artificial skeletal muscle tissues by using magnetic force. Cell culture at 39°C promoted the differentiation of myoblast cells into myotubes. Moreover, the mild and transient heat treatment improved the contractile properties of artificial skeletal muscle tissue constructs. These findings indicate that the culture method using heat treatment is a useful approach to enhance functions of artificial skeletal muscle tissue.

Keywords: C2C12 cells, contractile force, heat treatment, magnetic force, skeletal muscle, tissue engineering.

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Article Details

VOLUME: 14
ISSUE: 13
Year: 2013
Page: [1083 - 1087]
Pages: 5
DOI: 10.2174/1389201015666140408125231
Price: $58

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