Abstract
The present study deals with the green synthesis of silver nanoparticles (AgNPs) using Citrus reticulata juice as the reducing agent. The reaction carried out at room temperature (25°C) and at 55°C. The AgNPs were characterized by UV-Vis, TEM, XRD, AFM and FTIR measurements. The produced AgNPs were also evaluated for their increased antibacterial activities with tetracycline and penicillin against Gram-positive and Gram-negative bacteria. In this study, combination of AgNPs (at 55°C) and penicillin showed high inhibition zone in E. coli (18 mm) compared to that of AgNPs (at 25°C) and tetracycline. The cytotoxic effects of AgNPs from the Citrus reticulate juice on B16/ F10 melanoma cell line were evaluated by MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) assay. MTT assay showed a minimum cell inhibition (14.83%) at 0.125 mM (AgNPs at 25°C) and a maximum cell inhibition (31.29%) was observed at 1 mM (AgNPs at 55°C).
Keywords: Green synthesis, Citrus reticulata juice, AgNPs, antibacterial studies, cytotoxicity.
Current Nanoscience
Title:Green Synthesis of Silver Nanoparticles Using Citrus Reticulata Juice and Evaluation of their Antibacterial Activity and Cytotoxicity Against Melanoma-B16/F10 Cells
Volume: 9 Issue: 4
Author(s): T.V.M. Sreekanth, P. C. Nagajyothi, T.N.V.K.V. Prasad and Kap Duk Lee
Affiliation:
Keywords: Green synthesis, Citrus reticulata juice, AgNPs, antibacterial studies, cytotoxicity.
Abstract: The present study deals with the green synthesis of silver nanoparticles (AgNPs) using Citrus reticulata juice as the reducing agent. The reaction carried out at room temperature (25°C) and at 55°C. The AgNPs were characterized by UV-Vis, TEM, XRD, AFM and FTIR measurements. The produced AgNPs were also evaluated for their increased antibacterial activities with tetracycline and penicillin against Gram-positive and Gram-negative bacteria. In this study, combination of AgNPs (at 55°C) and penicillin showed high inhibition zone in E. coli (18 mm) compared to that of AgNPs (at 25°C) and tetracycline. The cytotoxic effects of AgNPs from the Citrus reticulate juice on B16/ F10 melanoma cell line were evaluated by MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) assay. MTT assay showed a minimum cell inhibition (14.83%) at 0.125 mM (AgNPs at 25°C) and a maximum cell inhibition (31.29%) was observed at 1 mM (AgNPs at 55°C).
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Cite this article as:
Sreekanth T.V.M., Nagajyothi C. P., Prasad T.N.V.K.V. and Lee Duk Kap, Green Synthesis of Silver Nanoparticles Using Citrus Reticulata Juice and Evaluation of their Antibacterial Activity and Cytotoxicity Against Melanoma-B16/F10 Cells, Current Nanoscience 2013; 9 (4) . https://dx.doi.org/10.2174/1573413711309040007
DOI https://dx.doi.org/10.2174/1573413711309040007 |
Print ISSN 1573-4137 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6786 |
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