Abstract
Free radical nitric oxide (NO) has been known to interact with various physiological processes, such as wound repair processes and control of vascular tone. However, NO is an unstable molecule and the development of NO delivery systems that enhance its stability has also been studied. In this work, alginate/chitosan nanoparticles have been studied as a drug delivery system of the Snitrosoglutathione (GSNO) as NO donor. For this, glutathione, GSH, the GSNO precursor, was encapsulated in alginate/chitosan nanoparticles. The presence of GSNO was confirmed by UV spectra at 336 nm. Alginate/chitosan nanoparticles with negative and positive surface charges were obtained by increasing the chitosan amount. The encapsulation efficiency (EE) relied on the nanoparticle zeta potential, obtaining 80% of EE for positive particles. The NO release from GSNO showed that polymeric nanoparticles lead to the stabilization of GSNO decomposition, at physiological temperature. Moreover, this system did not exhibit cytotoxicity for fibroblast V79 cells up to the maximum concentration tested (18 μmolL-1). These results showed that alginate/chitosan nanoparticles are interesting particles to encapsulate NO donors for biomedical applications where NO might have a therapeutic effect.
Keywords: Polymeric nanoparticles, alginate, chitosan, nitric oxide, glutathione, cytotoxicity, s-nitrosoglutathione, pharmacology
Current Nanoscience
Title:Development of a Sustained-release System for Nitric Oxide Delivery using Alginate/Chitosan Nanoparticles
Volume: 9 Issue: 1
Author(s): Priscyla D. Marcato, Leonardo F. Adami, Raquel de Melo Barbosa, Patricia S. Melo, Iasmin R. Ferreira, Larissa de Paula, Nelson Duran and Amedea B. Seabra
Affiliation:
Keywords: Polymeric nanoparticles, alginate, chitosan, nitric oxide, glutathione, cytotoxicity, s-nitrosoglutathione, pharmacology
Abstract: Free radical nitric oxide (NO) has been known to interact with various physiological processes, such as wound repair processes and control of vascular tone. However, NO is an unstable molecule and the development of NO delivery systems that enhance its stability has also been studied. In this work, alginate/chitosan nanoparticles have been studied as a drug delivery system of the Snitrosoglutathione (GSNO) as NO donor. For this, glutathione, GSH, the GSNO precursor, was encapsulated in alginate/chitosan nanoparticles. The presence of GSNO was confirmed by UV spectra at 336 nm. Alginate/chitosan nanoparticles with negative and positive surface charges were obtained by increasing the chitosan amount. The encapsulation efficiency (EE) relied on the nanoparticle zeta potential, obtaining 80% of EE for positive particles. The NO release from GSNO showed that polymeric nanoparticles lead to the stabilization of GSNO decomposition, at physiological temperature. Moreover, this system did not exhibit cytotoxicity for fibroblast V79 cells up to the maximum concentration tested (18 μmolL-1). These results showed that alginate/chitosan nanoparticles are interesting particles to encapsulate NO donors for biomedical applications where NO might have a therapeutic effect.
Export Options
About this article
Cite this article as:
D. Marcato Priscyla, F. Adami Leonardo, de Melo Barbosa Raquel, S. Melo Patricia, R. Ferreira Iasmin, de Paula Larissa, Duran Nelson and B. Seabra Amedea, Development of a Sustained-release System for Nitric Oxide Delivery using Alginate/Chitosan Nanoparticles, Current Nanoscience 2013; 9 (1) . https://dx.doi.org/10.2174/1573413711309010003
DOI https://dx.doi.org/10.2174/1573413711309010003 |
Print ISSN 1573-4137 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6786 |
Call for Papers in Thematic Issues
Role of nanomaterials in fabrication of coatings, Machining and Joining
The application of nanoscience has brought about a revolution in the field of mechanical engineering by providing novel materials, boosting manufacturing processes, and generating cutting-edge products. The purpose of this special issue is to investigate the enormous impact that nanoscience has had on mechanical engineering, with a particular emphasis on ...read more
Advanced Inorganic Nanocomposites and Their Emerging Applications
This special issue collection will highlight developments on the recent trends about the synthesis of metal oxides, nanoclusters, biomaterials, 2D nanomaterials, nanocrystals, nanocomposites, etc. and their applications in electrochemical systems, tissue regeneration, energy storage and harvesting, sensors, etc. The novelty of the methods in the chemical synthesis and their characterizations, ...read more
Applicability of Nanotechnology for Performance Enhancement of Clean Energy Technologies
Population growth, industrialization, and improvement in living quality would lead to further increase in energy demand in near future. Regarding the disadvantages of fossil fuels such as fluctuations in their price, high emissions of greenhouse gases and restriction of their sources, it is crucial to use and exploit alternative energy ...read more
Graphene and 2D Materials for Energy Storage and Conversion
This thematic issue will discuss the recent advances in graphene-based nanomaterials for different energy technologies. Graphene possesses a high surface area, and stable structure and exhibits many interesting electronic, optical, and mechanical properties due to its 2D crystal structure. Graphene is of both fundamental interest and suitable for a wide ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Therapeutic Potential of Autotaxin/Lysophospholipase D Inhibitors
Current Drug Targets Inhibition of IL-2 Production by Novel Small Molecules using Building Blocks from Reduced Chalcones and a Substituted Proline
Current Drug Therapy Synthesis, Molecular Docking and Anticancer Evaluation of New Arylazothiazoles
Current Organic Synthesis Antineoplastic Activity, Structural Modification, Synthesis and Structure-activity Relationship of Dammarane-type Ginsenosides: An Overview
Current Organic Chemistry Polymeric Nanocarriers for the Oral Delivery of Bioactives
Current Drug Therapy Evaluation of a Reporter Gene Assay for Bioactivity Determination of Therapeutic Interferons through a Collaborative Study
Current Pharmaceutical Analysis Contingency Management for Smoking Cessation: Where Do We Go from Here?
Current Drug Abuse Reviews G-Quadruplex Based Probes for Visual Detection and Sensing
Current Pharmaceutical Design Luminescent Lanthanide Labels
Current Inorganic Chemistry (Discontinued) Anti-Inflammatory Properties of Drugs from saffron crocus
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Aryl/heteroaryl Substituted Celecoxib Derivatives as COX-2 Inhibitors: Synthesis, Anti-inflammatory Activity and Molecular Docking Studies
Medicinal Chemistry Improvement of Bioactive Potential of Canavalia Beans of Coastal Sand Dunes by Solid-Substrate Fermentation Using Rhizopus oligosporus
Current Nutrition & Food Science Modulation of Signaling Enhances the Efficacy of the Combination of Satraplatin and Erlotinib
Current Drug Targets New Cathepsin D Inhibitorswith Hydroxyethylamine Isosteres: Preparation and Characterization
Medicinal Chemistry Synthesis, In Vitro Hydrolysis, Bioanalytical Method Development and Pharmacokinetic Study of an Amide Prodrug of Ibuprofen
Current Pharmaceutical Analysis Cannabinoids: Novel Medicines for the Treatment of Huntingtons Disease
Recent Patents on CNS Drug Discovery (Discontinued) Metabolomic Approach in Probing Drug Candidates
Current Topics in Medicinal Chemistry Therapeutic Targeting of TRP Channels - The TR(i)P to Pain Relief
Current Topics in Medicinal Chemistry Design, Synthesis and Biological Evaluation of 4-(Imidazolylmethyl)-2- Aryl-Quinoline Derivatives as Aromatase Inhibitors and Anti-breast Cancer Agents
Letters in Drug Design & Discovery Bio-AIMS Collection of Chemoinformatics Web Tools based on Molecular Graph Information and Artificial Intelligence Models
Combinatorial Chemistry & High Throughput Screening