Abstract
Brevibacillus expression system is an effective bacterial expression system for secretory proteins. The host bacterium, Brevibacillus choshinensis, a gram-positive bacterium, has strong capacity to secrete a large amount of proteins (∼30g/L), which mostly consist of cell wall protein. A host-vector system that utilizes such high expression capacity has been constructed for the production of secretory proteins and tested for various heterologous proteins, including cytokines, enzymes, antigens, and adjuvants.
Keywords: Brevibacillus choshinensis, host-vector system, secretory production, recombinant protein, gram-positive bacteria
Current Pharmaceutical Biotechnology
Title: Brevibacillus Expression System: Host-Vector System for Efficient Production of Secretory Proteins
Volume: 11 Issue: 3
Author(s): Makoto Mizukami, Hiroshi Hanagata and Akira Miyauchi
Affiliation:
Keywords: Brevibacillus choshinensis, host-vector system, secretory production, recombinant protein, gram-positive bacteria
Abstract: Brevibacillus expression system is an effective bacterial expression system for secretory proteins. The host bacterium, Brevibacillus choshinensis, a gram-positive bacterium, has strong capacity to secrete a large amount of proteins (∼30g/L), which mostly consist of cell wall protein. A host-vector system that utilizes such high expression capacity has been constructed for the production of secretory proteins and tested for various heterologous proteins, including cytokines, enzymes, antigens, and adjuvants.
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Cite this article as:
Mizukami Makoto, Hanagata Hiroshi and Miyauchi Akira, Brevibacillus Expression System: Host-Vector System for Efficient Production of Secretory Proteins, Current Pharmaceutical Biotechnology 2010; 11 (3) . https://dx.doi.org/10.2174/138920110791112031
DOI https://dx.doi.org/10.2174/138920110791112031 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
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