miR-4319 Suppresses the Growth of Esophageal Squamous Cell Carcinoma Via Targeting NLRC5

(E-pub Ahead of Print)

Author(s): Xiao Hu, Min Wang, Lei Cao, Li Cong, Yujie Gao, Jianwei Lu, Jifeng Feng, Bo Shen, Delin Liu*.

Journal Name: Current Molecular Pharmacology

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Background: The functions of microRNAs (miRNAs) in cancer progression have been recognized in recent years. However, the role of miR-4319 in esophageal squamous cell carcinoma (ESCC) remains unclear.

Objective: We aimed to investigate the biological roles of miR-4319 in ESCC progression and the associated mechanisms.

Methods: Real-time PCR was performed to examine the levels of miR-4319 in ESCC cell lines. The effects of miR-4319 and NOD-like receptor (NLR) family, caspase activation and recruitment domain (CARD) domain containing 5 (NLRC5) on cell proliferation and cell cycle progression were evaluated using MTT assay, colony formation and flow cytometry assays. Bioinformatics techniques and luciferase reporter assay were applied to validate NLRC5 as a miR-4319 target.

Results: miR-4319 expression was lower in ESCC cells than in normal cell line. Force the expression of miR-4319 repressed cell growth and induced cell cycle arrest. NLRC5 was validated as a direct downstream target of miR-4319. Overexpression of NLRC5 potentiated the effects of miR-4319 on cell growth and cell cycle distribution.

Conclusion: Our results demonstrated that miR-4319 might function as a tumor suppressor by targeting NLRC5 in ESCC.

Keywords: miR-4319, NLRC5, Esophageal squamous cell carcinoma, Cell growthl, Cell cycle

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Article Details

(E-pub Ahead of Print)
DOI: 10.2174/1874467212666191119094636
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