Background: The functions of microRNAs (miRNAs) in cancer progression have been recognized
in recent years. However, the role of miR-4319 in esophageal squamous cell carcinoma
(ESCC) remains unclear.
Objective: We aimed to investigate the biological roles of miR-4319 in ESCC progression and the associated
Methods: Real-time PCR was performed to examine the levels of miR-4319 in ESCC cell lines. The
effects of miR-4319 and NOD-like receptor (NLR) family, caspase activation and recruitment domain
(CARD) domain containing 5 (NLRC5) on cell proliferation and cell cycle progression were evaluated
using MTT assay, colony formation and flow cytometry assays. Bioinformatics techniques and luciferase
reporter assay were applied to validate NLRC5 as a miR-4319 target.
Results: The miR-4319 expression was lower in ESCC cells than in the normal cell line. The expression
of miR-4319 repressed cell growth and induced cell cycle arrest. NLRC5 was validated as a direct
downstream target of miR-4319. Overexpression of NLRC5 potentiated the effects of miR-4319 on cell
growth and cell cycle distribution.
Conclusion: Our results demonstrated that miR-4319 might function as a tumor suppressor by targeting
NLRC5 in ESCC.